Combination of Chemically-Characterized Essential Oils from , and : Optimization of a New Complete Antibacterial Formulation Using Simplex-Centroid Mixture Design.

This study aims to identify the volatile profile of three essential oils obtained from (EPEO), (OMEO), and (LBEO) and to examine their combined antibacterial activity that affords the optimal inhibitory ability against and using simplex-centroid mixture design and checkerboard assay. Essential oils (EOs) were isolated by hydrodistillation and characterized using gas chromatography-mass spectrometry (GC-MS) and gas chromatography coupled with flame-ionization detector (GC-FID). The antibacterial activity was performed using disc diffusion and microdilution assays.