Isolation, characterization, and analysis of pure compounds from leaf extract for antibacterial, antidiabetic, cytotoxicity and phytotoxicity.

This research aims to isolate, characterize, and analyze pure compounds from leaf extract to investigate its antibacterial, antidiabetic, cytotoxic, and phytotoxic effects. Fresh leaves were collected, dried, and subjected to methanol extraction to obtain a crude extract. From the petroleum ether fraction (PEF) of this extract, three fractions-designated LM1, LM2, and LM3-were prepared using column chromatography.

Analysis of bioactive compounds present in different crude extracts of and seeds by gas chromatography-mass spectrometry.

Plant seeds are the resources of many different bioactive components. The chemical composition of the different crude extracts from (White pumpkin) and (Pumpkin) seeds with three different polarity-based solvents (n-hexane, n-hexane-chloroform (2:1), and methanol) was analyzed to identify the biologically active compounds. Each of the extracts was analyzed by gas chromatography-mass spectrometry.