Evaluation of Phenotypic Tests for Carbapenemase Detection in Enterobacteriaceae in Tunisia.

Resistance to carbapenems in Enterobacteriaceae is a challenge for public health. Carbapenemase production is the leading mechanism. This work aims to evaluate four phenotypic methods for carbapenemase detection in comparison with a molecular method.

Evaluation of PCR -restriction fragment length polymorphism and PCR amplification of genomic regions of difference for the identification of strains in lymph nodes cultures.

Background: A rapid accurate identification of is essential for surveillance purposes.

Objectives: A PCR -Restriction Fragment Length Polymorphism (RFLP) and a multiplex PCR based on the detection of 3 regions of difference (RD-PCR): RD9, RD4 and RD1 were evaluated for the identification of in lymph nodes cultures, in Tunisia, during 2013-2015.

Methods: Eighty-two complex strains were identified using the biochemical tests, GenoType MTBC assay, PCR -RFLP and RD-PCR.