- Cryptosporidiosis, caused by the protozoan parasite Cryptosporidium, can lead to serious diarrhea, especially in immunocompromised individuals, and is a significant global public health concern.
- The study aimed to create a new real-time PCR method combined with high-resolution melting (HRM) analysis to detect and distinguish the less-studied Cryptosporidium species C. meleagridis alongside the more common C. hominis and C. parvum.
- Findings revealed that the developed qPCR-HRM assay effectively identifies and differentiates between these three species, demonstrating sensitivity and potential for genotyping Cryptosporidium infections.