Identification of a microbial sub-community from the feral chicken gut that reduces colonization and improves gut health in a gnotobiotic chicken model.

A complex microbial community in the gut may prevent the colonization of enteric pathogens such as . Some individual or a combination of species in the gut may confer colonization resistance against . To gain a better understanding of the colonization resistance against , we isolated a library of 1,300 bacterial strains from feral chicken gut microbiota which represented a total of 51 species.

A mutant fitness compendium in Bifidobacteria reveals molecular determinants of colonization and host-microbe interactions.

Bifidobacteria commonly represent a dominant constituent of human gut microbiomes during infancy, influencing nutrition, immune development, and resistance to infection. Despite interest as a probiotic therapy, predicting the nutritional requirements and health-promoting effects of Bifidobacteria is challenging due to major knowledge gaps. To overcome these deficiencies, we used large-scale genetics to create a compendium of mutant fitness in ().