Sex differences alter primitive progenitors in the C57BL/6 Tet2 knockout mouse model.

The precancerous expansion of hematopoietic cells, termed clonal hematopoiesis (CH), has been correlated to disease development and all-cause mortality. Despite multiple observations that hematopoietic stem cell and progenitors (HSPCs) are significantly affected by both sex and age, there remain few studies quantifying male and female HSPC populations in wild-type and transgenic Tet2 models over time. Here we determine that male mice (with a hematopoietic deficiency of Tet2 and control) have more LinSca-1c-kit (LSK) cells, that include multi-potent progenitor cells (MPP; LSK CD48-CD150-) and long-term hematopoietic stem cells (LT-HSC; LSK CD48-CD150) compared to females.