Publications by authors named "Samaneh Bolourchi"
IL-13-induced goblet cell metaplasia contributes to airway remodeling and pathological mucus hypersecretion in asthma. miRNAs are potent modulators of cellular responses, but their role in mucus regulation is largely unexplored. We hypothesized that airway epithelial miRNAs play roles in IL-13-induced mucus regulation.
View Article and Find Full Text PDFAccurate and informative microbiological testing is essential for guiding diagnosis and management of pneumonia in patients who are critically ill. Sampling of tracheal aspirate (TA) is less invasive compared with mini-bronchoalveolar lavage (mBAL) and is now recommended as a frontline diagnostic approach in patients who are mechanically ventilated, despite the historical belief that TA was suboptimal due to contamination from oral microbes. Advancements in metagenomic next-generation sequencing (mNGS) now permit assessment of airway microbiota without a need for culture and, as such, provide an opportunity to examine differences between mBAL and TA at a resolution previously unachievable.
View Article and Find Full Text PDFArticle Synopsis
- * Researchers used metagenomic next-generation sequencing (mNGS) on tracheal aspirates from 92 critically ill patients to develop predictive models that can distinguish harmful pathogens from harmless microbes and noninfectious issues.
- * The developed models achieved high accuracy in identifying LRTI-positive patients and could help improve diagnostic protocols by combining pathogen detection, microbiome analysis, and host gene expression metrics.