Publications by authors named "Sam Balasch"
Article Synopsis
- RNA-Seq analysis of human semen highlights the need for purifying sperm samples to remove somatic cells with excessive RNA, which can skew results.
- The study evaluated the effects of density gradient centrifugation on porcine sperm transcriptome, finding similarities between purified and non-purified samples, but also significant differences in gene expression levels.
- A total of 7,519 protein coding genes were identified, with 372 showing altered RNA levels post-purification, mainly linked to lower gene expression involved in translation and metabolism, suggesting the purification process impacts certain genes primarily from epididymal and germinal origins.
Circular RNAs (circRNAs) are emerging as a novel class of noncoding RNAs which potential role as gene regulators is quickly gaining interest. circRNAs have been studied in different tissues and cell types across several animal species. However, a thorough characterization of the circRNAome in ejaculated sperm remains unexplored.
View Article and Find Full Text PDFUnderstanding the molecular basis of cell function and ultimate phenotypes is crucial for the development of biological markers. With this aim, several RNA-seq studies have been devoted to the characterization of the transcriptome of ejaculated spermatozoa in relation to sperm quality and fertility. Semen quality follows a seasonal pattern and decays in the summer months in several animal species.
View Article and Find Full Text PDFThe present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10 min light, 10 min rest and 10 min of further light (10-10-10 pattern). This pattern induced an intense and transient increase in the majority of motility parameters, without modifying sperm viability and acrosome integrity.
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