Effect of alginate nanoparticles on the immunogenicity of excretory-secretory antigens against acute toxoplasmosis in murine model.

Efficacy of alginate nanoparticles (Alg-NPs) as vaccine delivery for the excretory-secretory antigens (ESAs) against the virulent strain of Toxoplasma gondii was evaluated. Swiss albino mice were intraperitoneally immunized with three doses of either in vivo and in vitro-prepared ESA vaccines, 20 µg each, at 2-week intervals, then were challenged with 2500 tachyzoites of the RH HXGPRT (-) Toxoplasma gondii strain, four weeks later. Mice mortality, tachyzoite number in both peritoneal fluid and impression smear, and viability, ultrastructural tachyzoite changes, measuring immunological markers, and histopathological changes of both liver and spleen were studied, in comparison to alum adjuvanted ESAs and infected control subgroups.

Influence of temperature and salinity on the viability and infectivity of Giardia lamblia and Cryptosporidia parvum.

The present work was designed to assess the effect of different degrees of temperature and salinity on the viability and infectivity of G. lamblia and C. parvum at different storage time.

Co-agglutination (Co-A): a rapid test for the diagnosis of experimental trichinellosis.

Co-agglutination test (Co-A test) which has a wide application spectrum in bacterial diseases has been used for the detection of some parasitic infections. This work was to evaluate Co-A test as a new diagnostic tool in detection of T. spiralis antigens in both sera and urine of experimentally infected mice as compared with ELISA, for the diagnosis of trichinellosis.