Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco.

Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilm-forming capacity in MRSA.

Correlation between staphylococcal biofilm formation in vitro and potential for catheter-related infections.

Introduction: The present study evaluated biofilm-forming capacity and the presence of both icaA and icaD genes among staphylococcal strains isolated from catheter-related infections and blood culture.

Methodology: Ninety staphylococcal isolates, which included 45 strains of catheter infection origin and 45 strains of blood culture origin, were tested for their ability to produce biofilm using microtiter test plates and a catheter test. The presence of icaA and icaD genes was determined by polymerase chain reaction (PCR).