Publications by authors named "Salut Botella"
Article Synopsis
- Increasing consumption of organic or ready-to-eat foods raises concerns about foodborne disease outbreaks, requiring effective detection methods for pathogens.
- Traditional microbiological culture techniques for identifying these pathogens are time-consuming and costly, leading researchers to utilize faster methods like multiplex PCR (mPCR).
- This study successfully developed a simple protocol that uses buffered peptone water (BPW) for enriching food samples, allowing for the effective detection of multiple pathogens simultaneously through mPCR, which streamlines and reduces the resources needed for food safety analysis.
The aim of this work was to assess the effect of chlorine water treatment on Arcobacter butzleri and to study the survival strategies of this organism in chlorinated and non-chlorinated drinking water. A. butzleri NCTC 12481 was inoculated into chlorinated and non-chlorinated water and samples were removed aseptically, immediately and periodically during the next 2 days (for chlorinated drinking water) or 35 days (for non-chlorinated drinking water).
View Article and Find Full Text PDFThe aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media.
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