Publications by authors named "Salome Asanidze"
Article Synopsis
- The study investigates how certain agents (LPA, PMA, A23187) trigger the exposure of phosphatidylserine (PS) on red blood cells (RBCs) by exploring three key mechanisms: scramblase activation, PKCα activation, and lipid flip flop.
- Researchers used flow cytometry and specific inhibitors to assess the impact on PS exposure and intracellular calcium levels after RBC treatment.
- Findings indicate that inhibiting scramblase and PKCα significantly reduces PS exposure, with the effect being stronger when both pathways are inhibited; also, blocking the Gardos channel similarly lowers PS exposure across all treatments.
Background/aims: The increase of the intracellular Ca2+ content as well as the exposure of phosphatidylserine (PS) on the outer cell membrane surface after activation of red blood cells (RBCs) by lysophosphatidic acid (LPA) has been investigated by a variety of research groups. Carrying out experiments, which we described in several previous publications, we observed some discrepancies when comparing data obtained by different investigators within our research group and also between batches of LPA. In addition, we found differences comparing the results of double and single labelling experiments (for Ca2+ and PS).
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