Publications by authors named "Sally Maddah"
Article Synopsis
- NMR flow devices allow for real-time analysis of metabolic profiles in living cells, but distinguishing between intracellular and extracellular signals has been a significant challenge.
- This study successfully used a flow tube system and diffusion NMR to quantitatively measure both intracellular and extracellular metabolites in human fibroblasts, identifying 35 metabolites in total.
- The method demonstrated sensitivity to changes in metabolite concentrations and metabolic variations under different culture conditions, suggesting potential applications in studying metabolic disorders in fibroblasts.
Background: Because of the interplay between mitochondrial respiration and cellular metabolism, the simultaneous monitoring of both cellular processes provides important insights for the understanding of biological processes. NMR flow systems provide a unique window into the metabolome of cultured cells. Simplified bioreactor construction based on commercially available flow systems increase the practicability and reproducibility of bioreactor studies using standard NMR spectrometers.
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