Publications by authors named "Salinthip Jarusintanakorn"
Chinese hamster ovary (CHO) cells represent the most preferential host cell system for therapeutic monoclonal antibody (mAb) production. Enhancing mAb production in CHO cells can be achieved by adding chemical compounds that regulate the cell cycle and cell survival pathways. This study investigated the impact of ectoine supplementation on mAb production in CHO cells.
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- The study examined how adenosine and its derivative, cordycepin, affect the production of the monoclonal antibody adalimumab in two CHO cell lines with different amplification systems.*
- Results indicated that while adenosine slowed cell growth and enhanced cell cycle activity, it optimally improved antibody production at a concentration of 1 mM on day 2, especially in GS-KO CHO cells in the absence of glucose.*
- Cordycepin required much higher doses in CHO-DHFR cells to show effects on growth, but only increased antibody production in cultures without glucose, highlighting its variable efficiency compared to adenosine.*
Determination of Chinese hamster ovary (CHO) cell densities and antibody titers from small volumes of cell culture supernatants using multivariate analysis and partial least squares regression of UV-Vis spectra.
Anal Bioanal Chem
September 2021
Antibody titer and viable cell density (VCD) are two important parameters that need to be closely monitored during the process of cell line development and manufacturing of therapeutic antibodies. Typically, determination of each parameter requires 10-100 μL of supernatant sample, which is not suitable for small scale cultivation. In this study, we demonstrated that as low as 2 μL of culture supernatants were sufficient for the analysis using UV-Vis spectrum assisted with partial least squares (PLS) model.
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