Cationic and anionic detergent buffers in sequence yield high-quality genomic DNA from diverse plant species.

Because of the heterogeneity among seedlings of outbreeding species, the use of seedling tissues as a source of DNA is unsuitable for the genomic characterization of elite germplasms. High-quality DNA, free of RNA, proteins, polysaccharides, secondary metabolites, and shearing, is mandatory for downstream molecular biology applications, especially for next-generation genome sequencing and pangenome analysis aiming to capture the complete genetic diversity within a species. The study aimed to accomplish an efficient protocol for the extraction of high-quality DNA suitable for diverse plant species/tissues.

Effectiveness of Augmentative Biological Control of UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against .

To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong inhibitory effects against . UAE2 and UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only , however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD).