Publications by authors named "Salim Makni"
The circadian clock plays a critical role in regulating plant physiology and metabolism. However, the way in which the clock impacts the regulation of lipid biosynthesis in seeds is partially understood. In the present study, we characterized the seed fatty acid (FA) and glycerolipid (GL) compositions of pseudo-response regulator mutants.
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- Plant de novo fatty acid synthesis occurs in plastids, starting with the production of acetyl-CoA from pyruvate via the pyruvate dehydrogenase complex (PDH), requiring the cofactor lipoic acid.
- The enzymes involved in lipoic acid biosynthesis are octanoyltransferase (LIP2) and lipoyl synthase (LIP1), with specific mutants affecting lipid and fatty acid profiles in seeds and seedlings.
- Analysis using gas chromatography and mass spectrometry showed that octanoyltransferase mutants exhibited altered fatty acid compositions, with a more significant impact in the LIP2p2 isoform mutant, indicating its role in regulating fatty acid synthesis in seeds.