Effect of thyroxine administration on the expression of epidermal growth factor in the kidney and submandibular gland of neonatal mice. An immunocytochemical and in situ hybridization study.

The effect of T4 administration on epidermal growth factor (EGF) expression in kidney and submandibular glands (SMG) was studied in newborn mice. EGF messenger RNA (mRNA) abundance and EGF immunoreactivity were assessed by in situ hybridization and immunohistochemical techniques, respectively. T4 treatment on days 0-6 augmented both the in situ hybridization and immunocytochemical signals in kidney but not in SMG on day 7.

Expression of epidermal growth factor in the kidney and submandibular gland during mouse postnatal development. An immunocytochemical and in situ hybridization study.

Earlier work has demonstrated that the salivary glands and kidneys are the major sites of epidermal growth factor (EGF) synthesis in adult mice. The precise timing of the onset of endogenous EGF synthesis in these tissues is not yet clear. In the present study we assessed the ontogenesis of EGF expression in the Swiss-Webster mouse.

Steroid sulfatase expression in human placenta: immunocytochemistry and in situ hybridization study.

Steroid sulfatase (STS), an important enzyme in the pathway of estrogen synthesis from sulfated steroid precursors, was localized to the syncytial trophoblast of human placentas during different periods of pregnancy by using a mouse monoclonal antibody and immunocytochemical techniques. Preembedding immunoelectron microscopy revealed STS immunoreactivity associated with the rough endoplasmic reticulum of the syncytial trophoblast. STS mRNA was also localized to this outermost layer of the human trophoblast.

Monoclonal analysis of fine-needle aspiration biopsy in kidney allografts.

To evaluate changes in T-lymphocyte subsets and DR expression on tubular cells, 74 fine-needle allograft aspirates (FNAB) were evaluated in 31 patients with cadaver kidney transplants. Monoclonal antibodies against T helper CD4+, cytotoxic/suppressor CD8+, and HLA-DR were used with an indirect alkaline-phosphatase-staining technique. Cases with acute rejection (n = 11) showed a significant increase of CD8+: CD4+ ratio versus those with stable function (n = 21), acute tubular necrosis (n = 10) or CsA toxicity (n = 7) (ANOVA F = 10; P less than 0.

Recovery of testicular blood flow following ligation of testicular vessels.

To determine whether initial ligation of the testicular vessels of the high undescended testis followed by a delayed secondary orchiopexy is a viable alternative to the classical Fowler-Stephens procedure, a series of preliminary experiments were conducted in the rat in which testicular blood flow was measured by the 133xenon washout technique before, and 1 hour and 30 days after ligation of the vessels. In addition, testicular histology, and testis and sex-accessory tissue weights were measured in 6 control, 6 sham operated and 6 testicular vessel ligated rats 54 days after vessel ligation. The data demonstrate that ligation and division of the testicular blood vessels produce an 80 per cent decrease in testicular blood flow 1 hour after ligation of the vessels.

In situ hybridization of prepro-epidermal growth factor mRNA in the mouse kidney.

The presence of prepro-epidermal growth factor (prepro-EGF) mRNA was studied in the mouse kidney by in situ hybridization using [3H]prepro-EGF cDNA and 35S-labeled prepro-EGF cRNA probes. In addition, anti-EGF serum was utilized to immunolocalize the peptide by the avidin-biotin complex immunoperoxidase method. Both EGF immunoreactivity and prepro-EGF mRNA hybridization were localized to the thick ascending limb of Henle (TAL) and the distal convoluted tubule (DCT), whereas the macula densa was negative.

Epidermal growth factor and the kidney.

Current information indicates that the mammalian kidney is a significant site of EGF synthesis, second only to the salivary gland in the rodent and probably exceeding most other tissues in the human species. The prepro EGF mRNA is localized to the cells of the TALH and the DCT. The EGF mRNA transcript in kidney is similar to that in salivary gland; the molecular mass of the prepro EGF protein in kidney approximates 130,000 kDa.

In situ hybridization of nerve growth factor mRNA in the mouse submandibular gland.

We studied the presence of beta-nerve growth factor (NGF) mRNA in the submandibular gland of the mouse by in situ hybridization using 35S-labeled prepro-beta-NGF antisense RNA. Female and male mice were studied at different stages of postnatal development, ranging from 3 to 12 weeks. Although NGF mRNA was detectable in the granular convoluted tubules of the submandibular gland in all the age and sex groups studied, the abundance of the signal dramatically increased after 5 weeks during the development of the submandibular gland.

Lack of prenatal testosterone surge in fetal rats exposed to alcohol: alterations in testicular morphology and physiology.

Pregnant Sprague-Dawley rats were administered a liquid alcohol diet (35% ethanol-derived calories), a pair-fed isocaloric diet, or dry food pellets beginning on Day 14 of gestation and continuing until parturition. Testosterone levels in male fetuses were measured on Days 17 through 20 of gestation. The normal surge of testosterone on Days 18 and 19 was present in controls, but notably absent in male fetuses exposed to alcohol.

Nerve growth factor immunoreactivity in mouse kidney: an immunoelectron microscopic study.

We have recently reported the immunolocalization of nerve growth factor (NGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of NGF by the preembedding immunoperoxidase method for electron microscopy. NGF immunoreactivity was present in the connecting tubule cells of the distal nephron.

Lymphangioma of the duodenum: an ultrastructural study.

Two cases of duodenal lymphangioma were diagnosed by endoscopy, and light and electron microscopic findings are reported. Ultrastructural studies showed smooth muscle cells around lymphatic vessels, and amyelinic nerve fibers and smooth muscle cells in the interstitium. On the basis of the ultrastructural features, a hamartomatous rather than tumoral origin is suggested for the duodenal lymphangioma.

Immunocytochemical localization of epidermal growth factor in mouse kidney.

Epidermal growth factor (EGF) was originally isolated from mouse submandibular glands (SMG). However, SMG removal failed to lower circulating EGF, and large amounts of EGF have been found in mouse urine. In addition, the presence of pre-pro-EGF mRNA in mouse kidney has recently been reported by others.

Immunoelectron microscopy of epidermal growth factor in mouse kidney.

We recently have reported the immunolocalization of epidermal growth factor (EGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of EGF by the preembedding immunoperoxidase method for electron microscopy. EGF immunoreactivity was present on the apical plasma membrane of the cells of the thick ascending limb of Henle and the distal convoluted tubule.

Endocervical polyp with pseudosarcomatous pattern and cytoplasmic inclusions: an electron microscopic study.

A case of endocervical polyp with atypical stromal cells in a 32-year-old woman is reported. Light microscopy showed atypical stromal cells with eosinophilic cytoplasmic inclusions which, by electron microscopy, were nonmembrane-bound, compact fibrillary structures in close relationship with the cytoplasmic microfibrils. The ultrastructural features demonstrated a fibroblastic nature of bizarre cells.

Immunocytochemical localization of nerve growth factor in mouse kidney.

The presence of NGF in mouse kidney was investigated using immunocytochemical methods. Female and male adult Swiss-Webster mouse kidneys were fixed by perfusion with 4% paraformaldehyde or Zamboni's fixative. The kidneys were frozen, and serial sections were prepared.