Sperm vitrification : influence of diluent, stored volume and supplementation with sulfated polysaccharides of Nile tilapia () skin.

The aim of this study was to evaluate the influence of diluent, stored volume and the cryodiluent medium supplementation with sulfated polysaccharides (SP) extracted from Nile tilapia skin on vitrified sperm. Six pools were diluted in 5% Glucose or Powder Coconut Water (ACP-104), supplemented or not with 0.5 mg/mL of SP, and submitted to vitrification.

Comparative ovarian morphophysiology of rats and Zebrafish after exposure to nandrolone decanoate.

This study aimed to compare the effects of nandrolone decanoate on the morphology and physiology of ovarian tissues in two experimental models, Zebrafish and rats, after in vitro cultivation. A total of 136 animals were used ( rats, n=36, and Zebrafish, n=100). In both experiments, the animals were divided into two groups (Control and Deca) and were exposed to nandrolone decanoate for seven weeks.

Supplementation of sulfate polysaccharides in the seminal cooling medium of common curimata (Prochilodus brevis).

Background: The use of sulfated polysaccharides (PS) in seminal cooling is known to improve seminal quality.

Objective: To evaluate the effect of different concentrations of PS, extracted from the macroalgae Gracilaria domigensis as a supplement to the seminal cooling medium of the reophilic fish Prochilodus brevis (common curimatã).

Materials And Methods: Five semen pools were diluted in ACP-104 (treatment T1), in BTS® (T2) and in BTS® with different concentrations of PS (0.

Sulfated polysaccharides from seaweed as a supplement to Prochilodus brevis sperm freezing medium.

Background: Using sulfated polysaccharides (SP) in fish sperm freezing medium promotes cell maintenance.

Objective: To evaluate the effect of different SP concentrations, extracted from two seaweeds (Gracilaria domingensis and Ulva fasciata), as a supplement to the sperm freezing medium of Prochilodus brevis.

Materials And Methods: Five semen pools were diluted in a solution composed of 5% glucose, 10 % dimethyl sulfoxide (DMSO) and different SP concentrations (0, 0.

The effect of glycosaminoglycans, extracted from the skin of tilapia, in the sperm freezing medium of Colossoma macropomum.

Background: Sulfated polysaccharides from the skin of Nile tilapia (Oreochromis niloticus), added to the tambaqui (Colossoma macropomum) semen diluting medium, can be potential antioxidants and promote the maintenance of sperm quality.

Objective: To evaluate the use of different concentrations of glycosaminoglycans (GAGs) from the skin of Nile tilapia as a supplement in two cryogenic media for tambaqui semen.

Materials And Methods: Tambaqui males received a single dose of pituitary carp extract.

[Analysis of medical short-term admissions of elderly patients with focus on alternatives to hospitalization].

By a retrospective medical record review it is studied if 334 admissions of acute short-term hospitalization (< 24 hours) of elderly medical patients could have been substituted by alternative solutions. 27% of these admissions were evaluated as substitutable. This study cannot confirm that the number of medical short-term admissions of elderly patients could be reduced by one or more specific interventions, nor that the medical short-term hospitalization generally is inappropriate.

Acute admissions to medical departments. A comparison between an urban and a rural district.

To compare hospitalization into medical departments, acute admissions into a city hospital and into a district hospital were compared prospectively over a two-week period. Patients referred to the city hospital were on average older, were more frequently living alone and they had a greater amount of social care attendance in their homes. On the other hand, distribution of referral diagnoses, overall patient activity, occupational status and contact with relatives were similar in the two areas.

Quantitative high-performance liquid chromatographic, gas chromatographic, and gas chromatographic-mass spectrometric analysis of ticlopidine in baboon plasma after solid-phase extraction.

High-performance liquid chromatography with UV detection (HPLC-UV) and gas chromatography with either nitrogen phosphorus (GC-NPD) or mass spectrometry (GC-MS) detection were used for the determination of ticlopidine in plasma. Solid-phase extraction of ticlopidine from plasma was performed using Extrelut columns without pH adjustment, and using hexane as the solvent of elution. With HPLC, a mobile phase of 0.

Circulating and brain metabolites of minaprine in the baboon.

A mixture of 15N-labelled, 14C-labelled and unlabelled minaprine was administered orally to three baboons, and metabolites in blood, urine and brain investigated. Biological samples were extracted with dichloromethane and the radioactive components extracted were analysed by t.l.

Metabolism of ethyl loflazepate in the rat, the dog, the baboon and in man.

The metabolism of ethyl loflazepate (Victan), a new benzodiazepine used as an anxiolytic drug, was studied in man and several animal species. The urinary metabolites were determined and quantified following oral administration of the 14C-labeled drug. Further analysis of blood samples was carried out in order to get information on the circulating metabolites in man.

Analysis of the metabolites of ethyl loflazepate by gas chromatography with electron-capture detection.

A gas chromatographic assay with electron-capture detection (GC--EC) is described for the metabolites of ethyl loflazepate (Victan), a new benzodiazepine with a potent anti-anxiety activity, in biological fluids. Since the parent drug undergoes a first-pass effect, pharmacokinetic data may only be obtained by measuring the total levels of two of the major metabolites. Accurate data can not be obtained for the metabolites separately since one of them (M1) is chemically transformed to the other (M2) during plasma sampling, storage and extraction.