Neuronal Cell Types in the Spinal Trigeminal Nucleus of the Camel Brain.

Neurons in the spinal trigeminal nucleus of a camel were morphologically studied by the Golgi impregnation method. The neurons were classified based on the size and shape of their cell bodies, the density of their dendritic trees, and the morphology and distribution of their appendages. At least 12 morphological types of neurons were found in the camel spinal trigeminal nucleus, including the following: stalked, islets, octopus-like, lobulated, boat-like, pyramidal, multipolar, round, oval, and elongated neurons.

A Golgi study of neurons in the camel cerebellum (Camelus dromedarius).

Neurons in the cerebellar cortex of camels were studied using modified Golgi impregnation methods. Neurons were classified according to their position, morphology of their soma, density and distribution of dendrites, and the course of their axons. Accordingly, eight types of neurons were identified.

Neuronal cell types in the anterior ventral thalamic nucleus of the camel.

The anterior ventral nucleus neurons in of the camel brain were morphologically studied by Golgi impregnation method. Two neuronal types of were found in the camel anterior ventral thalamic nucleus, namely, Golgi-type I neurons and Golgi-type II neurons. Those neurons were generally similar to their counterparts in the human thalamus.

Fracture resistance of fixed partial dentures supported by different abutment combinations: an ex vivo study.

This study sought to compare the fracture resistance of and categorize failure modes exhibited by fixed partial dentures (FPDs) supported by natural teeth, implants, or mixed tooth-implant abutments. Three groups utilized three different abutment combinations: all natural teeth, all implants, and a mixture of teeth and implants. The teeth were prepared and zero-angled titanium abutments were installed over the implants.

A Golgi study of the camel cuneate nucleus.

Neurons in the cuneate nucleus of the camel brain stem were studied by Golgi method. Six types of neurons were identified based on soma size and shape, density of dendritic trees, morphology and distribution of spines, and appendages. Type I neurons had large spherical somata with somatic appendages.

Recommendations on: internal standard criteria, stability, incurred sample reanalysis and recent 483s by the Global CRO Council for Bioanalysis.

"The Global CRO Council (GCC) for Bioanalysis was formed in an effort to bring together many CRO leaders to openly discuss bioanalysis and the regulatory challenges unique to the outsourcing industry"

Two-way crossover bioequivalence study of alendronate sodium tablets in healthy, non-smoking male volunteers under fasted conditions.

This study was conducted in order to assess the bioequivalence of two different formulations containing 70 mg alendronate sodium (CAS 121268-17-5) under fasted conditions. One hundred twenty-two healthy male volunteers were enrolled in an open label, randomized, crossover design with a wash-out period of 20 days in one study center. Urine samples were collected up to 36 h post-dose, and the concentrations of alendronic acid were determined using a high performance liquid chromatographic method with pre-derivatization and fluorescence detection (HPLC/FL) method.

Bioanalytical method development and validation using incurred samples--simultaneous quantitation of ramipril and ramiprilat in human EDTA plasma by LC-MS/MS.

A new method development and validation approach is proposed in order to develop a reliable method for the simultaneous quantitation of ramipril and ramiprilat in the presence of numerous labile metabolites. This new approach involves the usage of a synthesized labile acyl glucuronide of ramipril as well as individual and pooled incurred (study) samples in the development and validation process. Following the method validation and prior to its application to a large clinical study, a mini pilot study was performed to evaluate the performance of the method.

Internal standard response variations during incurred sample analysis by LC-MS/MS: Case by case trouble-shooting.

Internal standard (IS) responses can directly impact the accuracy of reported concentrations in bioanalysis as the majority of LC-MS/MS methods are based on analyte/IS response ratios for quantitation. Due to the complexity of incurred sample matrices and drug formulation, variable IS responses are quite common upon applying a validated method to the analysis of incurred samples. To maintain the integrity of a study and to avoid economic losses, it is therefore extremely important to monitor IS response variations during bioanalysis and to quickly identify the root causes if variations are observed.

Automated liquid-liquid extraction method for high-throughput analysis of rosuvastatin in human EDTA K2 plasma by LC-MS/MS.

Background: Liquid-liquid extraction has been widely used for the analysis of rosuvastatin due to its many attractive features, such as low cost and clean extract. However, manual transfer of the organic phase poses a challenge, particularly when a batch size is large. To overcome the challenge, a simple automated high-throughput (192 samples per batch) liquid-liquid extraction method with short (3.

The 2nd Calibration and Validation Group workshop on recent issues in good laboratory practice bioanalysis.

This event was organized by the Calibration and Validation Group (a scientific nonprofit organization based in Toronto, Canada) as a 1.5-day workshop for contract research organizations and pharmaceutical companies involved in providing bioanalytical data for bioavailability, bioequivalence, pharmacokinetic and comparability studies.

Bioequivalence studies of tacrolimus capsule under fasting and fed conditions in healthy male and female subjects.

The bioequivalence of tacrolimus (CAS 104987-11-3) 5 mg capsules was assessed in two single-dose, open-label, randomIzed 2-way crossover trials with a minimum washout period of 14 days; one trial was conducted under fasting condition (n = 44) and the other one under fed condition (n = 48). Blood samples were collected over a 120-h period and concentrations were assayed using a liquid chromatography tandem mass spectrometry (LCMS/MS) method. A non-compartmental method was used for calculation of pharmacokinetic parameters.

Prevalence of mistreatment and justice of grading system in five health related faculties in Jordan University of Science and Technology.

Background: Many Jordanian university students complain of the behavior of some teaching staff. Also, they complain of the grading systems in universities.

Aim: This study concerns the occurrence of different forms of student mistreatment and student mistrust of the grading system in the Jordan University of Science and Technology (JUST) as an example of universities in Jordan.

Complex neurovascular variation in one upper limb.

Variations in the arterial pattern of the upper limb are frequently encountered during dissection of the human body. However, multiple variations in one limb are infrequently encountered. Dissection of the left amputated upper limb, 15 cm above the intercondyler line, of a female cadaver revealed the following variations.

An evaporation-free solid-phase extraction method for rapid and accurate analysis of sumatriptan in human plasma by LC-MS/MS.

An evaporation-free solid-phase extraction (SPE) method was developed and validated for sumatriptan. High organic washing (50% methanol) and low organic elution (20% methanol) were used and the recovery was greater than 92%. The eluate was injected into a C18 column without evaporation and reconstitution.

Effects of orchids () on reproductive function and fertility in adult male mice.

The aim of the present study was to determine the effect of orchid bulbs on the reproductive system of male albino mice. Orchid bulb extract was fed to 20 male albino mice (5 g/mouse/day) for 35 days and compared with a similar number of mice as controls. Total testicular germ cell population, histometrical parameters, serum blood biochemistry and hormonal assay were determined.