Development of a Simple, Fast, and Cost-Effective Nanobased Immunoassay Method for Detecting Norovirus in Food Samples.

This study presents a quick, low-cost, and easy technique for the detection of norovirus in several food samples, including cucumber, lettuce, and chicken. The developed sandwich immunoassay method depends on employing nanotechnology for the detection step. Lactoferrin immobilized on activated Q-tips cotton swabs was used as a general capturing reagent to bind viruses from the sample surface.

Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria.

Cotton swab is the conventional swabbing tool that is usually applied for collecting pathogens from contaminated surfaces, followed by cells lysis and DNA extraction before subjecting to genetic analysis. However, such an approach is time consuming as it involves several steps and requires highly trained personnel to perform the experiment. In this study, we developed a new cotton swab-based detection system that involved integrating bacterial collection, preconcentration and detection on Q-tips.

A rapid colorimetric immunoassay for the detection of pathogenic bacteria on poultry processing plants using cotton swabs and nanobeads.

The work describes a simple cotton swab-based colorimetric immunoassay as a rapid screening tool for pathogenic bacteria on poultry processing plants. This immunosensing platform can be used for the detection of pathogens present on surfaces such as glass, stainless steel and chicken meat. Unlike the reported assays, here, cotton swab plays dual function: as a sample collector from the solid surfaces and as detection platform.

Fluorometric graphene oxide-based detection of Salmonella enteritis using a truncated DNA aptamer.

The work describes a fluorescence-based study for mapping the highest affinity truncated aptamer from the full length sequence and its integration in a graphene oxide platform for the detection of Salmonella enteriditis. To identify the best truncated sequence, molecular beacons and a displacement assay design are applied. In the fluorescence displacement assay, the truncated aptamer was hybridized with fluorescein and quencher-labeled complementary sequences to form a fluorescence/quencher pair.

Monitoring pesticide residues in dates marketed in Al-Qassim, Saudi Arabia using a QuEChERS methodology and liquid chromatography-tandem mass spectrometry.

A sensitive, simple and rapid QuEChERS extraction method and liquid chromatography equipped with triple quadrupole mass spectrometry (LC-MS/MS) were used to determine 42 pesticides in dates. Acidified acetonitrile and citrate buffer salts were used to extract re-hydrated samples. Acceptable validation performances were achieved, i.

Concentrations of trace elements [corrected] and organochlorines in Mutton bird (Puffinus griseus).

The Sooty Shearwater (Puffinus griseus, commonly known as Mutton bird) is a migratory wild seabird, annually harvested for food by certain native groups in New Zealand and Australia and in many parts of the world. The concentrations of 22 elements and several organochlorine pesticides [2,2-bis(chlorophenyl)-1,1,1-trichloroethane (DDT), its derivatives dichlorodiphenyldichloroethene (DDE) and dichlorodiphenyldichloroethane (DDD), aldrin, chlordane, dicofol, lindane, and methoxychlor] in Mutton bird were determined over two consecutive years to evaluate its safety for human consumption. Twenty bird carcasses were purchased in each of 2007 and 2008 from a local source.