Coupling the GAL4 UAS system with alcR for versatile cell type-specific chemically inducible gene expression in Arabidopsis.

The Aspergillus alc regulon encodes a transcription factor, ALCR, which regulates transcription from cognate promoters such as alcA(p). In the presence of suitable chemical inducers, ALCR activates gene expression from alcA(p). The alc regulon can be transferred to other species and can be used to control the expression of reporter, metabolic and developmental genes in response to low-level ethanol exposure.

CycD1, a putative G1 cyclin from Antirrhinum majus, accelerates the cell cycle in cultured tobacco BY-2 cells by enhancing both G1/S entry and progression through S and G2 phases.

A putative G1 cyclin gene, Antma;CycD1;1 (CycD1), from Antirrhinum majus is known to be expressed throughout the cell cycle in the meristem and other actively proliferating cells. To test its role in cell cycle progression, we examined the effect of CycD1 expression in the tobacco (Nicotiana tabacum) cell suspension culture BY-2. Green fluorescent protein:CycD1 is located in the nucleus throughout interphase.

Analysis of rotaviral gastroenteritis in Tbilisi.

Electrophoretypes of 107 rotaviral isolates collected in Tbilisi for 18 months revealed seven patterns from which 4 were "long" and 3 "short". The "long" electrophoretypes represented 74.8% of total number of the isolates analysed.

[Radioimmunologic analysis for the rapid diagnosis of arbovirus infections].

The employment of radioimmunoassay (RIA) allows arbovirus infections to be diagnosed at an early stage of the illness. In the present study the best variant of RIA was determined with regard to its practical use in rapid diagnosis of some arbovirus infections and its superiority over CFT, HI, IHA, IF. The method was shown to be highly specific and sensitive for qualitative studies of specimens from natural foci of arboviruses and for timely diagnosis of human illnesses.

[Cases of legionellosis infection in Tbilisi].

The epidemic outbreak of Legionella infection at two industrial enterprises during the period of July-August 1988 is described. The results of the epidemiological study, the clinical course and the serological diagnosis of the outbreak are presented. For the first time in the USSR a new form of the disease has been described at the territory of Georgia.

[The frequency of detection of markers of hepatitis virus B infection among the inhabitants of Rustavi, the Georgian SSR].

Blood serum samples from 1,087 patients with acute viral hepatitis were studied. HBsAg was detected in 36.6% of cases.

[Detection of natural foci of the hemorrhagic fever with renal syndrome in the Georgian SSR].

The circulation of the causative agent of hemorrhagic fever with renal syndrome (HFRS) in Georgia was studied. Altogether, 7,938 small mammals of 16 species trapped in 23 administrative areas of the republic were examined, and the specific antigen of HFRS virus was detected in 308 (3.8%).

[Etiological significance of rotaviruses in acute intestinal diseases in children].

The paper presents the results of virological, serological, and epidemiological examinations of patients with acute enteric infections of obscure etiology carried out for detection of rotavirus gastroenteritides. Rotaviruses have been found to play a certain role in generating acute gastroenteritides in infants under 3 years, mostly among infants, of 1-2 years, the least among those of 0-6 months of age. Adult family members were the source of infection for the children.

[Complex method for epizootiological and epidemiological surveillance].

A complex method for the epizootological and epidemiological surveillance of a number of bacterial and viral infections on the territories inside and outside their natural foci has been developed. The investigation techniques are described and the data on the isolation rate of each causative agent in different geographical zones are presented. In the natural foci of plague and tularemia, as well as on the territories outside such foci, the causative agents of intestinal yersiniosis, pseudotuberculosis, salmonellosis, erysipeloid, staphylococci and streptococci, arena- and arboviruses have been isolated from the rodents and ectoparasites under study.

[Detection of the tularemia zoonosis in the territory of the Kartlian Plain].

The natural focus of tularemia was found to cover the whole territory of the Kartlian plain. Epizooty occurred mainly among common voles with the involvement of insectivorous voles. Hard ticks, gamasids and fleas infected with Francisella tularensis were detected.