Mapping of leaf water content using near-infrared hyperspectral imaging.

In this study, near-infrared hyperspectral imaging was applied to predict the water content of golden pothos (Epipremnum aureum) leaves, after which partial least squares regression (PLSR) analysis was performed to predict their averaged water content. The resulting predictive model was then applied to each single-pixel spectra in order to construct a water content image that could be used to evaluate the model's applicability to the single-pixel spectra through partial least squares score comparisons between the averaged spectra used for calibration and the single-pixel spectra. In the next phase, it was determined that a rebuilt PLSR predictive model based on the averaged spectra of an applicable pixel showed higher prediction accuracy than that of the original model.

Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12.

The psychrophilic yeast Glaciozyma antarctica demonstrated high antifreeze activity in its culture filtrate. The culture filtrate exhibited both thermal hysteresis (TH) and ice recrystallization inhibition (RI) properties. The TH of 0.

Cryoprotection and cryosterilization effects of type I antifreeze protein on E. coli cells.

To establish the effects of type I antifreeze protein (AFP) on E. coli cells, we have focused on the survival rate of the E. coli cells using type I AFP at various concentrations under rapid cooling conditions using liquid N2 at atmospheric or low pressure.

A novel, intracellular antifreeze protein in an antarctic bacterium, Flavobacterium xanthum.

One strain of Antarctic bacteria, Flavobacterium xanthum IAM12026, has a highly active antifreeze protein (AFP) in the intracellular space. The cell-free extract from strain IAM12026 after culturing at 4 degree C for 7 days in TSB medium, had activity of 0.04 degree C at a concentration of 0.