Publications by authors named "Sakiho Tanaka"
Article Synopsis
- - The study reveals that myogenic stem satellite cell activator HGF experiences nitration due to peroxynitrite, leading to its diminished binding to the receptor c-met, which disrupts muscle health as we age.
- - A specific rat anti-HGF monoclonal antibody, 1H41C10, effectively protects HGF from nitration at critical tyrosine sites (Y198 and Y250), maintaining its function to activate satellite cells.
- - The findings suggest that 1H41C10 could be significant in developing treatments for age-related muscle loss and conditions like sarcopenia by preventing HGF dysfunction.
Mechanical perturbation triggers activation of resident myogenic stem cells to enter the cell cycle through a cascade of events including hepatocyte growth factor (HGF) release from its extracellular tethering and the subsequent presentation to signaling-receptor c-met. Here, we show that with aging, extracellular HGF undergoes tyrosine-residue (Y) nitration and loses c-met binding, thereby disturbing muscle homeostasis. Biochemical studies demonstrated that nitration/dysfunction is specific to HGF among other major growth factors and is characterized by its locations at Y198 and Y250 in c-met-binding domains.
View Article and Find Full Text PDFGenetic alterations in human epidermal growth factor receptor type 2 (HER2)/epidermal growth factor receptor (EGFR) are commonly associated with breast and lung cancers and glioblastomas. Cancers with avian erythroblastosis oncogene B (ERBB) deregulation are highly metastatic and can cause primary brain tumors. Currently, no pan-ERBB inhibitor with remarkable brain penetration is available.
View Article and Find Full Text PDFA pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF.
Biochem Biophys Rep
September 2022
Protein tyrosine residue (Y) nitration, a post-translational chemical-modification mode, has been associated with changes in protein activity and function; hence the accumulation of specific nitrated proteins in tissues may be used to monitor the onset and progression of pathological disorders. To verify the possible impact of nitration on postnatal muscle growth and regeneration, a pilot study was designed to examine the nitration/dysfunction of hepatocyte growth factor (HGF), a key ligand that is released from the extracellular tethering and activates myogenic stem satellite cells to enter the cell cycle upon muscle stretch and injury. Exposure of recombinant HGF (a hetero-dimer of α- and β-chains) to peroxynitrite induces Y nitration in HGF α-chain under physiological conditions.
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