Novel enzymatic method for assaying Lp-PLA in serum.

Background: Measurement of lipoprotein-associated phospholipase A (Lp-PLA) can be used as an adjunct to traditional cardiovascular risk factors for identifying individuals at higher risk of cardiovascular events. This can be performed by quantification of the protein concentration using an ELISA platform or by measuring Lp-PLA activity using platelet-activating factor (PAF) analog as substrate. Here, an enzymatic Lp-PLA activity assay method using 1-O-Hexadecyl-2-acetyl-rac-glycero-3-phosphocholine (rac C PAF) was developed.