[Immunological parameters in the assessment of the activity of a specific process in Mycobacterium tuberculosis-infected children and patients with intrathoracic lymphatic tuberculosis].

The data of a comprehensive study of 86 children aged 6 to 14 years, who were examined and treated at the Research Institute of Phthisiology for various manifestations of tuberculous infection: 25.6% with infected Mycobacterium tuberculosis with varying specific sensitization; 34.9% with minor forms of intrathoracic lymphatic tuberculosis (ITLT), 39.

[Some regularities of an immune response in patients with pulmonary tuberculosis and drug-resistant Mycobacterium strains].

Clinical and immunological studies were conducted in 62 patients with infiltrative pulmonary tuberculosis and 53 with disseminated one. As drug resistance and viability of mycobacteria increased, an immune response was found to develop as the humoral type and cellular immunity was suppressed. A more marked reduction in the activity of T helper cells type 1 of an immune response and the neutrophilic granulocytic system was revealed in patients with disseminated pulmonary tuberculosis.

[Estimation of the specific sensitization in a child and other immunological parameters in tuberculosis of intrathoracic lymph nodes during therapy].

The data of a comprehensive study of 86 children aged 6 to 14 years, who were examined and treated at the Research Institute of Phthisiology for various manifestations of tuberculous infection: 25.6% with infected Mycobacterium tuberculosis with varying specific sensitization; 34.9% with minor forms of intrathoracic lymph node tuberculosis (ILNT), and 39.

Kinetic models of cyclooxygenase and peroxidase inactivation of prostaglandin-H-synthase during catalysis.

Kinetic models of inactivation of cyclooxygenase and peroxidase activities of prostaglandin-H-synthase (PGHS) during cyclooxygenase and peroxidase reactions catalyzed by the enzyme and also on preincubation with H2O2 have been developed; these models account for data obtained by the authors as well as data from the literature. Being rather simple, these models simultaneously describe the processes of cyclooxygenase and peroxidase inactivation of PGHS, using the minimal set of experimental parameters.

[Frequently ill children's physical development].

The changes in the physical development of frequently ill children (FIC) aged 3-7 years are considered. The rate of an increase in the height and body weight of FIC has been found to differ from that of these indices in occasionally ill children (OIC). By the age of 7 years, FIC are statistically smaller than OIC of the same age.

[Immunological parameters and mycobacterial biological properties in infiltrative pulmonary tuberculosis].

Thirty-one patients with infiltrative pulmonary tuberculosis were clinically and immunologically examined. A relationship was found between the definite immunological parameters and the biological properties of mycobacteria. The higher viability of mycobacteria and the increase in the drug resistance have been shown to be associated with the decreased activity of lymphocytes and the suppressed production of interleukin-2, which suggests a decrease in the activity of type 1 T helper cells and a significant increase in the synthesis of tuberculosis antibodies with the lower serum concentrations of circulating immune complexes.

[Impact of physical exercises on the cardiovascular and respiratory systems of students].

The paper deals with the impact of physical classes at a higher educational establishment on the cardiovascular and respiratory systems of students. First- and second-year students who did physical exercises in accordance with the curriculum and fourth-year students who did not were followed up. Blood pressure and heart rate before and after exercise and the maximum oxygen consumption were used as parameters that characterized the state of the cardiovascular and respiratory systems.

[Cytological substantiation of using organosilicon sorbent gentaxan it the treatment of wound infections].

The main principle in the management of festering wound is the clearance of nidus of infection and so the methods of sorptive therapy are given considerable attention nowadays. We have used a new organosilicon sorbent gentaxan in the treatment of 45 woman recently confined with festering wounds. The obtained findings suggest that the use of gentaxan in treatment of such patients contribute to eliminating factors which hampers wound healing and thus it reduces the term of wound repair by 4-5 days.

[Parameters of acquired immunity and cation proteins of neutrophilic granulocytes in pulmonary tuberculosis].

Clinical and immunological studies of 61 patients with pulmonary tuberculosis have indicated that the status of acquired and congenital (natural) immunity assessed by the intra- and extracellular levels of cation proteins of neutrophilic granulocytes is different in different clinical forms of pulmonary tuberculosis. The found correlations suggest that there is a relationship between the factors of acquired and congenital immunity and that cytokines are involved in the regulation of specific inflammation. More active degranulation of azurophilic granules of neutrophils occurs due to myeloperoxidase.

[Immunological changes in patients with pulmonary tuberculosis during glutoxim therapy].

The paper deals with the effect of glutoxim included into a preoperative preparation regimen on immunological parameters in patients with fibrocavernous pulmonary tuberculosis. On admission, all the patients had inadequate cellular immunity and activated humoral immunity. After termination of a course of glutoxim therapy, there was an increase in the baseline low values of lymphocytic proliferative activity, in the count of mature T lymphocytes, and in the production of IL-2 induced by phytohemagglutinin.

Substrate specificity of african oil palm tree peroxidase.

The optimal conditions for catalysis by the peroxidase isolated from leaves of African oil palm tree (AOPTP) have been determined. The pH optimum for oxidation of the majority of substrates studied in the presence of AOPTP is in the interval of 4.5-5.

Extremely high stability of African oil palm tree peroxidase.

A detailed kinetic study on thermal inactivation of African oil palm tree peroxidase (AOPTP) at different pHs has been carried out. The enzyme does not undergo inactivation over a broad range from pH 2 to 12 at ambient temperature. Complete inactivation of AOPTP is observed only at 70 degrees C and extremal pHs like <3.

[Cytokine production-adenosine deaminase activity relationship in pulmonary tuberculosis].

The serum activity of the enzyme adenosine desaminase (ADA) was studied in patients with infiltrative tuberculosis in relation to IL-1 beta, TNF-alpha, IL-2 productions, the magnitude of a lymphocytic proliferative response to PPD and PGA. There was an association of high ADA levels with the severity of a tuberculous process, with the least IL-2 production together with drastically increased IL1 beta and significant disorders in the TNF-alpha system. Moderate ADA increases reflect the regularly enhanced activity of immunocompetent cells in response to an infectious agent.

[Roncoleukin immunomodulation in pulmonary tuberculosis].

The effects of roncoleukine (recombinant human interleukin-2) on clinical and immunological parameters were studied in patients with pulmonary tuberculosis. Twenty five patients with fibrocavernous pulmonary tuberculosis steadily progressive during the therapy were examined. Roncoleukine caused no complications and its clinical effect was recorded in 80.

[Changes in the production of interleukin-1 beta, tumor necrosis factor-alpha and interleukin-2 in relation to the immunity in patients with pulmonary tuberculosis].

The specific features of production of IL-1 beta, TNF-alpha, IL-2 were studied in 74 patients with various forms of tuberculosis by taking into account the magnitude of an immunological response. Tuberculin, phytohemagglutinin, prodigiosine were used as inducers of the synthesis of cytokines. Heterodirection was found in the changes of elaboration of cytokines in similar immunological disorders in persons with different clinical forms of tuberculosis.

[Production of cytokines in different forms of pulmonary tuberculosis].

The relationship of the production of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) to the pattern and etiology was studied in patients with pulmonary tuberculosis (n = 74) and nontuberculous lung diseases (n = 28). There was an inverse correlation between the production of the proinflammatory cytokines IL-1 beta and TNF-alpha and the main T-cellular immunity IL-2. An exacerbation of a tuberculous process is accompanied by an increase in IL-1 beta and TNF-alpha productions and by a decrease in inducted IL-2 synthesis.

[Production of tumor necrosis factor-alpha and interleukin-1 beta in patients with pulmonary tuberculosis in relation to the course of a process and immunological features].

The production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) by peripheral mononuclears induced by prodigiosane, tuberculin, phytohemagglutinin and unduced and the serum levels of the above cytokines were studied. There was an relationship between the production of TNF-alpha in the patients' peripheral blood and the type and pattern of a tuberculosis process. The similar tendencies were found in the production of IL-1 beta.

[Distribution of histocompatibility antigens in patients with pulmonary tuberculosis depending on disease course and immune response pattern].

Genetic relationships with tuberculous process and immunity were investigated within immunogenetic study of 178 patients with active pulmonary tuberculosis. Tuberculosis occurrence seemed to be associated with phenotypical antigen Cw2. Unfavorable outcome of the disease was observed in the presence of HLA A9, A11, B14, B40, B41 antigens.

[Non-specific resistance of neutrophil granulocytes by cationic protein indices in the development of tuberculosis].

The paper gives cytochemical findings of the lysosomal cationic system of neutrophilic granulocytes in the blood of 188 patients with various pulmonary tuberculoses. In infiltrative pulmonary tuberculosis which was first detected, the decrease in the activity of the lysosomal cationic system of neutrophils was adequate to the extent and severity of the process. The permanent increase in the antimicrobial potential of neutrophilic granulocytes in patients with fibrous and cavernous pulmonary tuberculosis is a defense response of the body, however with constantly progressive tuberculosis, there is inhibition of this defense system, which becomes an unfavourable sign.

[Immunological criteria in the differential diagnosis of lung diseases].

Immunological examination performed in 292 patients with pulmonary diseases which presented difficulties for differential diagnosis has shown essential differences in immunological status of tuberculous, pneumonia and cancer patients. The fact that immunological abnormalities were related to X-ray characteristics of the lesion made it possible to develop each X-ray syndrome-specific criteria of distinguishing tuberculosis from other pulmonary lesions. The advance of the disease caused unidirectional changes in immunograms for various pulmonary lesions.

[Characteristics of immunity in patients with destructive pulmonary tuberculosis].

Parameters of systemic immunity, activity of cation proteins of neutrophilic granulocytes and individual blood serum proteins were studied in two groups of patients: with newly diagnosed (83) and chronic destructive pulmonary tuberculosis (105). Four levels of T-lymphocytes were established per their absolute content in 1 ul of the peripheral blood, which had different interconnection with cell response intensity to mitogenic PHA stimulation and the values of the lysosomal cation++ test in both groups of patients. Indications for the prescription of immune active preparations based upon the T-cell level and, when necessary, supplemented by information related to the values of BTR with PHA and BTR with PPD were developed for both groups of patients independently of hereditary consolidated serum proteins.

[Factors, determining the effectiveness of tryptophanase interaction with amino acids].

Inhibition of tryptophanase-catalyzed decomposition of S-(o-nitrophenyl)-L-cysteine by a variety of amino acids has been investigated. For amino acids similar to the natural substrate and for those having minimal steric requirements for the side chain, the linear correlation exists between-RTlnKi and side chain hydrophobicity. L-ornithine and L-arginine are anomalously potent inhibitors taking into account low hydrophobicity of their side chains.

Linear dichroism studies of tryptophanase and its quasisubstrate complexes oriented in polyacrylamide gel.

Tryptophanase from Escherichia coli was oriented in a compressed slab of polyacrylamide gel and its linear dichroism (LD) and absorption spectra have been measured. The free enzyme displays four LD bands at 305, 340, 425 and 490 nm. Two bands at 340 and 425 nm belong to the internal coenzyme-lysine aldimine.