Somatostatin-like immunoreactivity in the facial, glossopharyngeal and vagal lobes of the carp.

A large number of somatostatin (SRIF)-containing structures was demonstrated in the vagal and glossopharyngeal lobes of the carp by means of the indirect immunofluorescent method. The present study further showed that the facial lobe is devoid of these structures. These facts indicate that SRIF may have an important role in relaying viscerosensory information from the vagal and glossopharyngeal nerve but not from the facial nerve.

Demonstration of a substance P-like immunoreactivity in retinal cells of the rat.

The distribution of substance P (SP)-like immunoreactivity in the rat retina was investigated by immunohistochemistry. SP-positive cells were found throughout the retina. The majority of them were located in the proximal portion of the inner nuclear layer and the processes from these cells directed to the inner plexiform layer where they ramified, suggesting that SP-positive cells located in this region probably are amacrine cells.

Somatostatin in the auditory system of the rat.

The present study clearly disclosed that the auditory system of the neonatal rat contains a large number of somatostatin (SRIF)-positive structures. Among them, n. cochlearis dorsalis and ventralis, n.

On the afferent projections from some meso-diencephalic nuclei to n. raphe magnus of the rat.

Afferent sources from meso-diencephalon to n. raphe magnus (RM) were examined by the HRP method. Following HRP injection into RM, HRP-labeled cell clusters occurred in: (1) n.

Experimental and morphological studies of te noradrenaline innervations in the nucleus tractus spinalis nervi trigemini of the rat with special reference to their fine structures.

An attempt was made to elucidate the origins and fine structure of the noradrenaline (NA) terminals in the nucleus tractus spinalis nervi trigemini (Vsp) of the rat. The present study revealed that the NA in the Vsp is supplied from two NA neuron systems; bilateral locus coeruleus (LC) and medullary NA neuron group, on the grounds that: (1) two types of fluorescent fibers (fine and coarse) were identified in the Vsp; and (2) HRP injected in to the Vsp resulted in labeling of neurons of the bilateral LC and bulbal NA areas, particularly the ipsilateral A1 area. The fine structure of the NA terminals in the Vsp was investigated by a modified potassium permanganate fixation method.

Ascending and cerebellar non-serotonergic projections from the nucleus raphe magnus of the rat.

Non-serotonergic ascending and cerebellar projections of rat nucleus raphe magnus (RM) were disclosed using the horseradish peroxidase technique. The ascending bundle rose form rostral RM and was divided into two components; one ascended in raphe regions and the other in reticular formation. The former on innervated n.

Localization of vasoactive intestinal polypeptide and neurotensin immunoreactivities in the avian retina.

The distribution of vasoactive intestinal polypeptide (VIP)- and neurotensin (NT)-like immunoreactivities in the chicken retina was investigated by immunohistochemistry. VIP- and NT-positive cells were found throughout the chicken retina. The majority of them were located in the proximal portion of the inner nuclear layer and the processes from these cells directed to the inner plexiform layer where they ramified, suggesting that VIP- and NT-positive cells located in this region probably are amacrine cells.

Topographic organization of the projection from the forebrain subcortical areas to the hippocampal formation of the rat.

Using the technique of iontophoretic microinjection of horseradish peroxidase, the present study disclosed the complexity and high degree of the topographic organization in the forebrain subcortical afferents to the different regions of rat hippocampus, e.g. diagonal band, posterior (PH), dorsomedial and rostral lateral hypothalamic nuclei chiefly project to the rostrodorsal part (DRA) and caudal gyrus dentatus including CA3, the supramammillary area predominantly to the rostroventral area (VRA), the area lateral to PH to the DRA and VRA, substantia innominata and some thalamic nuclei (n.

Ascending components of the medial forebrain bundle from the lower brain stem in the rat, with special reference to raphe and catecholamine cell groups. A study by the HRP method.

The afferent connection of the medial forebrain bundle (MFB) arising from the lower brain stem have been investigated by means of horseradish peroxidase (HRP) with sensitive substrate, the injection was made iontophoretically into MFB at various levels. After injection of HRP into MFB, a significant number of HRP-labeled neurons were observed in the following structures of the lower brain stem: (1) raphe nuclear group, (2) locus coeruleus, (3) n. laterodorsalis tegmenti, (4) parabrachial area, (5) A1, A2, A4, A5 and A7 areas where noradrenaline-containing neurons were disseminated, (6) A8, A9 and A10 areas which contain dopamine neurons, (7) surrounding area of the fasciculus longitudinalis medialis at the level of the n.