[Disorders of the hormonal and immune status of patients with newly detected diabetes mellitus].

A study is presented of C-peptide, free and bound insulin, blood serum euglobulins, A, M, G immunoglobulins, circulating immune complexes, insulin antibodies and heterophilic hemagglutinins in 22 patients with newly diagnosed diabetes mellitus and in 29 healthy donors. It was established that at the early stage of diabetes mellitus the peripheral blood showed significant amounts of bound insulin as insulin-anti-insulin antibody complexes.

[Cytofluorimetric analysis of blood lymphocyte subpopulations detected using monoclonal antibodies in patients with type I diabetes mellitus].

The content of lymphocytes of different immunological phenotypes: T11+ (all T-lymphocytes), T8+ (suppressors/killers), T4+ (inducers/helpers) and NKH1+ (natural killer cells) was investigated in the blood of 11 patients with type I diabetes mellitus by the method of flow-rate cytofluorimetry using a laser cell sorting device EPICS-C (Coulter), and a kit of monoclonal antibodies; 16 primary donors entered into the control group. As compared to the controls the patients demonstrated a statistically significant decrease in the absolute content of T11+, T4+, T8+ and especially NKH1+-cells. The results obtained confirmed previous data reported by us and other authors concerning disorder in the system of NK cells in insulin dependent diabetes mellitus.

[The number and ultrastructure of large granule-containing lymphocytes (natural killer cells) in the blood of patients with diabetes mellitus type 1].

The content and ultrastructure of blood large granule containing lymphocytes (LGL) associated with the system of natural killer cells (NKC) were investigated in primary untreated patients with type I diabetes mellitus using light and electron microscopy. A marked decrease in the content of LGL and change in their submicroscopic structure indicating disorder of their function, were noted in the blood of patients with type I diabetes mellitus as compared to those in the blood of healthy subjects. LGL cytoplasm revealed a decrease in the number and size of specific electron dense granules (azurophil granules), their destruction and vacuolization were noted; the number of peculiar submicroscopic formations (parallel tubular structures) was on an increase.

[Anti-insulin antibodies and circulating immune complexes in patients with diabetes mellitus].

The authors presented the results of a study of the levels of circulating immune complexes and antiinsulin antibodies in patients with diabetes mellitus with regard to the main clinical characteristics of disease. The level of immune complexes was shown to reflect the gravity of disease and a degree of renal and reticular vascular lesion. This relationship was most noticeable in patients with type I and type II diabetes mellitus on insulin therapy.