Publications by authors named "Sakae Nagasawa"

Following the discovery of the primary culture of neural stem cells, the spheroid culture has been recognized as one of the selective culture methods for somatic stem cells. Since then, various methods were reported to generate spheroids, which can enrich the potent stem cell population. However, the fundamental factors affecting spheroid formation remain unclear.

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To construct a human teeth database which is freely available to researchers and students, three-dimensional human tooth models were generated in a previous study, by means of micro-CT, from 35 human teeth extracted during orthodontic treatment. In this study, X-ray images of 55 extracted human teeth were acquired using three-dimensional micro-CT at a resolution of 50x50x50 microm, and then visualized using a numerical data visualization software. These carious tooth models provided insight into the morphology and progression of carious defects as well as a rare insight into the morphology of carious tooth pulp, therefore rendering them as a useful tool and efficient method for dental students' learning.

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Osteoclasts are multinucleated cells that resorb bone. Although osteoclasts originate from the monocyte/macrophage lineage, osteoclast precursors are not well characterized in vivo. The relationship between proliferation and differentiation of osteoclast precursors is examined in this study using murine macrophage cultures treated with macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB (RANK) ligand (RANKL).

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With use of dental implants on the rise, there is also a tandem increase in the number of implant fracture reports. To the end of investigating the stress occurring in implants, elasticity and plasticity analyses were performed using the finite element method. The following results were obtained: (1) With one-piece type of implants of 3.

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The thermal expansion rate, coefficient of thermal expansion, and high temperature strength of two types of commercially available alloy for metal-bond porcelain, KIK-HII (KIK) and Degubond-J2 (J2), were evaluated up to the liquidus point temperature using a thermo-mechanical analyzer. Furthermore, microstructure in the solid-liquid coexisting region was observed for evaluation. Our results revealed the following findings: 1.

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Objective: This study investigated the effects of H(2)O(2)-treatment of titanium surfaces on cement shear bond strengths, and characterized H(2)O(2)-treated titanium surfaces.

Methods: Using 34.5% hydrogen peroxide solution, cp Ti plates (10 mm x 10 mm x 1 mm) were treated by (1) an immersion method, and (2) halogen irradiation while immersed in H(2)O(2) for varying times.

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Dolomite, a mineral composed of calcium magnesium carbonate (CaMg (CO3)2), is used as a food supplement that supplies calcium and magnesium. However, the effect of magnesium supplementation on bone metabolism in patients with osteoporosis is a matter of controversy. We examined the effects of daily supplementation with dolomite on calcium metabolism in ovariectomized (OVX) rats.

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This study evaluates effects of repeated baking processes on the mechanical and physical properties of single and triple applications of opaque, body and enamel porcelains fused to three different metal substrates (precious metal, semi-precious metal and non-precious metal). The vintage halo porcelain system was employed and fused to metals. Fused samples were subjected to three-point bend tests to evaluate bend strength and modulus of elasticity.

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Previously, high temperature properties of the silver-palladium-copper-gold alloy were investigated. In this study, the thermal expansion percentage and coefficient, and high temperature strengths of ADAS Type 3 gold alloy were investigated up to the liquidus temperature. Furthermore, microstructural and compositional changes in the solid/liquid dual phase were studied.

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Ag-Pd-Cu-Au alloy was subjected to a Thermo-Mechanical Analyzer to investigate high temperature properties up to its liquidus temperature. Microstructural examination and elemental analysis with EPMA were also conducted in the solid/liquid mixture region. The following conclusions were obtained.

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The success of the oral rehabilitation of implant patients depends not only on the osseointegration of implant fixtures but also on maintaining the integrity of the connection of prosthetic superstructures to these fixtures. It was an objective of the present study to evaluate and compare cement bond strengths among rolled (R), cast (C) and metal-injection-molded (M) commercially pure titanium plates which were bonded with Panavia 21 (Kuraray) and Imperva (Shofu) cements. Two plates (15x5x1 mm) of each R, C, and M were lap-jointed (lap length: 5 mm).

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Statement Of Problem: Dental casting accuracy is influenced by the setting expansion of investment materials. Although setting expansion can help compensate for casting shrinkage, it cannot be fully realized under a confined wax pattern. Exactly how soft a wax pattern should be to ensure optimum setting expansion has not been determined.

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