Publications by authors named "Sajid Fiaz"

The increasing temperature is a major threat to plant growth and development. It severely alters various biochemical and physiological processes and ultimately affects the overall crop yield. The membrane-based remorin protein-encoding genes (REM) were previously reported as significantly involved in the regulation of various biotic and abiotic stressors.

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Nitrogen (N) is a major plant nutrient and its deficiency can arrest plant growth. However, how low-N stress impair plant growth and its related tolerance mechanisms in peanut seedlings has not yet been explored. To counteract this issue, a hydroponic study was conducted to explore low N stress (0.

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Enhanced grain yield and quality traits are everlasting breeding goals. It is therefore of great significance to uncover more genetic resources associated with these two important agronomic traits. Plant MYB family transcription factors play important regulatory roles in diverse biological processes.

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Modern plant breeding technologies have played a central role in addressing global food security challenges. These technologies, including next-generation sequencing (NGS) and multi-omics analysis, genome-wide association analysis (GWAS), genome editing and transgenics, machine learning, and speed breeding, have been improving crop yield and quality as well as crop adaptability under climate change conditions, such as tolerance to both biotic and abiotic stresses. Furthermore, identification, searching, assessment, and combining desirable integrated (morphological, physiological, and biochemical) attributes have been achieved with greater accuracy, efficiency, time, and cost-effectiveness, all of which are essential to meeting global food demands.

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Climatic change and extreme weather events have become a major threat to global agricultural productivity. Plants coexist with microorganisms, which play a significant role in influencing their growth and functional traits. The rhizosphere serves as an ecological niche encompassing plant roots and is a chemically complex environment that supports the growth and development of diverse plant-interactive microbes.

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Article Synopsis
  • The study focuses on the relationship between root architecture and the Gibberellic acid-sensitive (GA-sensitive) Rht genes in wheat, particularly their role in improving drought tolerance and plant growth.
  • Out of 200 wheat genotypes analyzed under normal and osmotic stress conditions, significant differences were found in the number of seminal roots and overall root/shoot growth, indicating that genotypes with GA-sensitive Rht13 gene performed better than those with GA-insensitive Rht1 gene.
  • Specifically, certain genotypes showed enhanced root systems (up to 5 seminal roots) and improved growth metrics under stress, suggesting that the presence of GA-sensitive Rht genes is beneficial for nutrient uptake from deeper soil layers.
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Background: The bZIP transcription factor family, characterized by a bZIP domain, plays vital roles in plant stress responses and development. While this family has been extensively studied in various plant species, its specific functions in Camelina sativa (False Flax) remain underexplored.

Methods And Results: This study identified 71 bZIP transcription factors in C.

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  • - The CRISPR/Cas9 system has been applied to edit specific genes in HD and K326 tobacco cultivars, optimizing the system to target lignin and pectin synthesis genes (Ntab06050 and Ntab0857410).
  • - Eight constructs for lignin-related genes and five for pectin-related genes were created; targeting was guided by identifying PAM sequences for effective editing.
  • - Successful mutations of the targeted genes resulted in mutation frequencies of 6.2% for pectin-related NtabGAUT4 and 9.4% for lignin-related NtabCCoAMT.
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  • - In mid-latitude rice systems, mineral nitrogen fertilizers are crucial but lead to significant nitrogen losses, prompting the need for better soil organic matter management and sustainable practices to improve nitrogen efficiency and crop yields.
  • - A study evaluated the effects of combining mineral urea with organic fertilizers (farmyard manure and poultry compost) on various rice genotypes (NUYT-1 to NUYT-6) at Pakistan's National Agricultural Research Centre.
  • - Results showed that using poultry compost along with mineral urea notably increased nitrogen content in soil and plants, boosted shoot and root growth, and enhanced overall crop productivity, with an optimal N application strategy involving a mix of organic and mineral sources.
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CPSF30, a key polyadenylation factor, also serves as an mA reader, playing a crucial role in determining RNA fate post-transcription. While its homologs mammals are known to be vital for viral replication and immune evasion, the full scope of CPSF30 in plant, particular in viral regulation, remains less explored. Our study demonstrates that CPSF30 significantly facilitates the infection of turnip mosaic virus (TuMV) in , as evidenced by infection experiments on the engineered mutant.

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  • Plants face various biotic and abiotic stresses that hinder their growth and productivity, making micronutrient application essential for optimal yield, especially in leafy vegetables.
  • This study explores how different concentrations of copper sulfate (0, 75, 125, and 175 ppm) affect the growth and biochemical traits of Spinacia oleracea (spinach) and Avena sativa (oats).
  • The findings show that 75 ppm of copper sulfate boosts plant height, leaf area, and chlorophyll levels while higher concentrations, particularly 175 ppm, lead to decreased plant health and productivity, highlighting the importance of using optimal nutrient levels.
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  • * The study investigated the effects of biochar application on wheat varieties (FSD08 and PUNJAB-11) under salinity stress, using different sodium chloride levels and biochar concentrations.
  • * Results showed that biochar significantly mitigated the negative impacts of salt on growth and yield, and enhanced the plant's self-defense mechanisms, suggesting it as a beneficial amendment for sustainable crop production in saline conditions.
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Breeding high yielding water-deficit tolerant rice is considered a primary goal for achieving the objectives of the sustainable development goals, 2030. However, evaluating the performance of the pre-breeding-promising parental-lines for water deficit tolerance prior to their incorporation in the breeding program is crucial for the success of the breeding programs. The aim of the current investigation is to assess the performance of a set of pre-breeding lines compared with their parents.

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Farmers are using municipal wastewater either treated or untreated for irrigation because of limited fresh water resources. Wastewater extensively used for irrigation purposes is enriched with many nutrients. The reuse of wastewater is imposing a negative impact on human health and the ecosystem.

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  • Heavy metals like cadmium are harmful to both plants and people, and researchers are focusing on this issue.
  • Iron is really important for plants, but there's not enough of it in the soil because it often doesn't dissolve well.
  • A study showed that using FeO nanoparticles can help plants grow better even when they are exposed to cadmium, especially at a lower dose of 20 mg/L.
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The study was conducted to investigate the effect of green net shade during staggered planting times on growth, biochemical, antioxidant enzymes and vase life of gladiolus cut flowers. The green net shade effectively reduces the internal temperature, particularly during extremely hot planting times. Under the green net shade conditions, high quality morphological and biochemical observations were observed during the months of March and April planting times.

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Article Synopsis
  • Investigation Focus
  • : Researchers studied the roles of two genes, TaSnRK2.4 and TaSnRK2.9, in enhancing wheat's ability to withstand adverse environmental conditions and their potential applications in wheat breeding in Pakistan.
  • Methodology
  • : The team assessed genetic diversity using existing and newly developed markers, specifically KASP for TaSnRK2.4 and CAPS for TaSnRK2.9, but found limited variation in TaSnRK2.4 among local wheat varieties.
  • Key Findings
  • : The study highlighted the importance of haplotype Hap-5A-1 of TaSnRK2.9, which correlated with beneficial traits like increased productive tillers and grains per spike
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Global crop yield has been affected by a number of abiotic stresses. Heat, salinity, and drought stress are at the top of the list as serious environmental growth-limiting factors. To enhance crop productivity, molecular approaches have been used to determine the key regulators affecting stress-related phenomena.

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Background: Salinity is one of the main abiotic factors that restrict plant growth, physiology, and crop productivity is salt stress. About 33% of the total irrigated land suffers from severe salinity because of intensive underground water extraction and irrigation with brackish water. Thus, it is important to understand the genetic mechanism and identify the novel genes involved in salt tolerance for the development of climate-resilient rice cultivars.

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Drought is one of the main environmental stressors that can alter the water status of plants; negatively affect growth, assimilation, and photosynthesis; and eventually reduce crop yield. We explored the dependence of drought tolerance traits on chlorophyll-A content. Local sunflower cultivars (FH-01, FH-628, FH-633, FH-572, and FH-653) were grown in pots and subjected to drought by withholding water for 10, 15, or 20 d.

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Neurodegenerative disorders, caused by progressive neuron loss, are a global health issue. Among the various factors implicated in their pathogenesis, dysregulation of acetylcholinesterase activity has been recognized as a key contributor. Acetylcholinesterase breaks down the neurotransmitter acetylcholine, important for neural transmission.

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Article Synopsis
  • The study identified 30 genes in the soybean genome that help plant resilience against abiotic stresses, categorized into three phylogenetic groups.
  • Gene structure analysis revealed that some of these genes lack introns, and their protein products are localized in various cell compartments like the nucleus and mitochondria.
  • Expression profiling showed these genes are active in different plant parts and particularly upregulated in response to salt stress, with results validated through additional qRT-PCR analysis.
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Background: Millions of people around the globe are affected by Alzheimer's disease (AD). This crippling condition has no treatment despite intensive studies. Some phytocompounds have been shown to protect against Alzheimer's in recent studies.

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