Fluorescence Quenching of Graphene Quantum Dots from Orange Peel for Methyl Orange Detection.

Methyl orange (MO) is an organic synthetic dye widely used in laboratory and industrial applications. In laboratory settings, it serves as an acid-base indicator due to its distinct color change in both acidic and alkaline environments. Industrially, it is primarily utilized in the textile industry for its ultraviolet (UV) absorption properties.

Development of a method for the isolation and culture of astrocytes from the canine cerebral cortex.

Background: Astrocytes are considered key players in neuroimmunopathological processes, and they play a certain role in neuroinflammation. Rodent primary astrocyte cultures are commonly used in the study of human neuroinflammation. However, gene sequence homologies are closer between humans and dogs than between humans and rodents.

[Excessive fluoride increases the expression of osteocalcin in the mouse testis].

Objective: To observe the influence of excessive fluoride on the levels of osteocalcin and testosterone in the testis of the male mouse.

Methods: Twenty-four C57BL/6J male mice were equally randomized into a normal control and a fluorosis model group, the former fed on distilled water while the latter on a solution of sodium fluoride (100 mg/L) in distilled water, both for 12 weeks. Then, the level of osteocalcin in the testis tissue was measured with the immunohistochemical streptavidin-peroxidase (SP) method and those of osteocalcin and testosterone in the serum determined by ELISA.

A case-control study of rheumatoid arthritis revealed abdominal obesity and environmental risk factor interactions in northern China.

Background: The aim of this study was to evaluate new and previously hypothesized environmental risk factors and their interaction with rheumatoid arthritis (RA).

Methods: Four hundred patients recently diagnosed with RA and 400 controls frequency-matched by gender and birth year using Propensity Score Matching (PSM) were selected from northern China. Investigation was performed using self-reported data from interviewer-administered surveys.