Accurately Tunable AuNC-ZIF Content Architecture Based on Coordination-Dissociation Mechanism Enables Highly Brightness Dual-Site Fluorescent Biosensor.

The quantum yield and fluorescence intensity of gold nanocluster (AuNC) nanocarriers are critical parameters for developing ultrasensitive biosensors. In this study, AuNCs-zeolitic-imidazolate-framework (Au-ZIF) nanocomposites are systematically constructed by impregnating AuNCs onto the ZIF-8 surface through a coordination-dissociation mechanism, resulting in a dual-site fluorescence-loaded structure. In this configuration, AuNCs are anchored to the external surface while the integrity of the inner cavity remains intact.

A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of .

Tuberculosis caused by poses a global infectious threat to humans and animals. Therefore, there is an urgent need to develop a sensitive, precise, and easy-to-readout strategy. Here, a novel tandem combination of a CRISPR/Cas12a system with dual HCR (denoted as CRISPR/Cas12a-D-HCR) was constructed for detecting .

GmST1, which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3.

Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses.

miR‑26a inhibits ovarian cancer cell proliferation, migration and invasion by targeting TCF12.

Epithelial ovarian cancer (OC) is a common cause of death from gynecological tumors. MicroRNAs (miRNAs) may function as either oncogenes or tumor suppressors, playing crucial role not only in tumorigenesis, but also in tumor invasion and metastasis. miR‑26a and transcription factor 12 (TCF12) have been reported to be involved in carcinogenesis, but the regulatory role of miR‑26a/TCF12 in OC remains unknown.

TCF12 overexpression as a poor prognostic factor in ovarian cancer.

Introduction: Ovarian cancer is a common malignant tumor that is severely harmful to human health, but the molecular mechanisms of ovarian cancer remain unclear. Transcription factor 12 (TCF12) is a member of the basic helix-loop-helix (bHLH) E protein family, which recognizes the E-box sequence and is responsible for cellular development and differentiation. A recent study has reported that TCF12 is highly expressed in some human cancers and may be correlated with clinicopathological factors, but there are few studies on its mechanism.

Association of hCG and LHCGR expression patterns with clinicopathological parameters in ovarian cancer.

In addition to its critical role during pregnancy, human chorionic gonadotropin (hCG) has been shown to be expressed by various tumor types. Recent studies have similarly documented the presence of the luteinizing hormone (LH)/hCG receptor (LHCGR) in a variety of nongonadal organs; however, its clinicopathological significance in ovarian cancer remains unclear. The present study used a combination of immunohistochemical, real-time PCR, and western blot analyses to examine hCG and LHCGR expression in normal and cancerous tissues collected from patients with epithelial ovarian cancer (EOC).

Vasodilation effect of volatile oil from Allium macrostemon Bunge are mediated by PKA/NO pathway and its constituent dimethyl disulfide in isolated rat pulmonary arterials.

The present study aimed to investigate the vasodilation effects of Allium macrostemon Bunge (AMB) on isolated rat pulmonary arterials (PAs) and to assess the underling mechanisms. The volatile oil was extracted by steam distillation from the bulbs of AMB. Then the volatile oil from AMB was studied on isolated rat PA, removal of endothelium, or pretreatment with nitro oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME), or with protein kinase A (PKA) inhibitor PKI but not cyclooxygenase inhibitor indomethacin significantly blocked the AMB induced relaxation on PE-contracted PA rings.

Astragaloside IV protects rat gastric mucosa against aspirin-induced damage.

Aspirin (Asp) is commonly used as an anti-inflammatory drug, but the long-term usage of Asp can lead to severe gastrointestinal damage. Thus the co-administering of Asp with another drug that can suppress its side effect while having no impact on its anti-inflammatory activity would be ideal. Astragaloside IV (AST-IV) is a natural anti-inflammatory compound that has been shown to protect rat gastric mucosa from anhydrous ethanol-inflicted damage.

Effects of HCG on human epithelial ovarian cancer vasculogenic mimicry formation .

Ovarian cancer is the leading cause of mortality due to gynecological malignancy, and vasculogenic mimicry (VM) formation is correlated with poor prognosis. In a previous study, the present authors observed that human chorionic gonadotropin (HCG) could promote VM formation in three-dimensional OVCAR-3 cell cultures. In order to investigate whether HCG could promote VM formation in ovarian cancer , the role of OVCAR-3 cells overexpressing or depleted of chorionic gonadotropin, beta polypeptide 5 (CGB5, which is the fifth subunit of β-HCG and was identified as the key part of HCG) were injected into nude mice in the present study, while BeWo cells were used as a positive control.

Involvement of human chorionic gonadotropin in regulating vasculogenic mimicry and hypoxia-inducible factor-1α expression in ovarian cancer cells.

Background: Human chorionic gonadotropin (hCG) can play a crucial role in angiogenesis. In the present study, we focused on hCG to gain insight into its potential effects on vasculogenic mimicry (VM) in ovarian cancer cells.

Methods: Ovarian cancer OVCAR-3 cells were incubated with different concentrations of recombinant hCG in 3-dimensional cultures.

An HCG-rich microenvironment contributes to ovarian cancer cell differentiation into endothelioid cells in a three-dimensional culture system.

We investigated the expression of human chorionic gonadotropin (HCG) and its effects on vasculogenic mimicry (VM) formation in ovarian cancer cells under normoxic and hypoxic conditions in three-dimensional matrices preconditioned by an endothelial-trophoblast cell co-culture system. The co-culture model was established using human umbilical vein endothelial cells (HUVECs) and HTR-8 trophoblast cells in a three-dimensional culture system. The co-cultured cells were removed with NH4OH, and ovarian cancer cells were implanted into the preconditioned matrix.

A new peptide ligand for colon cancer targeted delivery of micelles.

Ligands are an imperative part of targeted drug delivery systems, and choosing a ligand with high affinity is a subject of considerable interest. In this study, we first synthesized a 12-residue peptide (TK) that interacts with integrin α6β1 overexpressed on colonic cancer cells. The molecular binding affinity assay indicated that TK had a high binding affinity for integrin α6β1.

Preparation and characterization of a novel aspirin derivative with anti-thrombotic and gastric mucosal protection properties.

The use of acetylsalicylic acid (ASP) is limited by its adverse effects, especially the effect on the gastric mucosa. To address this problem, we synthesized a derivative form of ASP, prepared by modification of ASP with nano-hydroxyapatite (a kind of inorganic particle containing Ca(2+)). The derivative was named Ca-ASP.

Iron and manganese removal by using manganese ore constructed wetlands in the reclamation of steel wastewater.

To reclaim treated steel wastewater as cooling water, manganese ore constructed wetland was proposed in this study for the removal of iron and manganese. In lab-scale wetlands, the performance of manganese ore wetland was found to be more stable and excellent than that of conventional gravel constructed wetland. The iron and manganese concentration in the former was below 0.