Reducing Pre- and Post-Treatments in Cryopreservation Protocol and Testing Storage at -80 °C for Norway Spruce Embryogenic Cultures.

Somatic embryogenesis (SE) is considered the most effective method for vegetative propagation of Norway spruce ( L. Karst). For mass propagation, a storage method that is able to handle large quantities of embryogenic tissues (ETs) reliably and at a low cost is required.

Norway spruce somatic embryogenesis benefits from proliferation of embryogenic tissues on filter discs and cold storage of cotyledonary embryos.

Vegetative propagation opens opportunities for the multiplication of elite tree progeny for forest regeneration material. For conifers such as Norway spruce () the most efficient vegetative propagation method is seed multiplication through somatic embryogenesis. Efficient culture methods are needed for somatic embryogenesis to be commercially viable.

Somatic Embryo Yield and Quality From Norway Spruce Embryogenic Tissue Proliferated in Suspension Culture.

Somatic embryogenesis is being piloted for the commercial production of genetically improved Norway spruce ( L. Karst) forest regeneration material in Finland. The main challenge to making the process commercially relevant is the dependence on time-consuming and highly skilled manual labor.

Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation.

Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication.

Somatic Embryogenesis and Plant Regeneration From Primordial Shoot Explants of (L.) H. Karst. Somatic Trees.

The recalcitrance of adult conifer tissues has prevented vegetative propagation of trees with known and desired characteristics. Somatic embryogenesis (SE) initiation protocol, recently developed for white spruce (, Klimaszewska et al., 2011), was applied in order to examine the feasibility, frequency and timing of SE induction from primordial shoots (PS) of Norway spruce ).

Reliable and practical methods for cryopreservation of embryogenic cultures and cold storage of somatic embryos of Norway spruce.

Somatic embryogenesis (SE) is considered as the most-effective method for vegetative propagation of Norway spruce (Picea abies L. Karst). For mass propagation, a cryopreservation method able to handle large numbers of embryogenic tissues (ETs) reliably and at low costs is needed.

Molecular phylogeny and systematics of anoplocephaline cestodes in rodents and lagomorphs.

A molecular phylogenetic hypothesis is presented for the anoplocephaline cestodes of placental mammals based on sequence data from the mitochondrial cytochrome c oxidase I (COI) gene, the nuclear-encoded 28S rRNA gene and the internal transcribed spacer region I of rRNA (ITS1). The material consists of 35 species representing nine genera of cestodes, with emphasis on taxa parasitising rodents and lagomorphs in the Holarctic region. The resulting phylogenies show considerable disagreement with earlier systematic and phylogenetic hypotheses derived from morphology.