Publications by authors named "Saikusa H"

Background: Rapid progressive encephalopathy with a high fever, consciousness loss and recurrent convulsions has been occasionally reported in children during influenza pandemics in Japan since 1995. We examined a 2-year old girl with hemorrhagic shock and encephalopathy syndrome associated with acute influenza A virus infection (A/Nagasaki/76/98; H3N2), to answer several questions for which no histologic or virologic data exist.

Methods: A clinicopathologic study using immunohistochemical staining and viral genome detection by reverse transcriptase polymerase chain reaction (RT-PCR) was performed with this autopsied case.

View Article and Find Full Text PDF

The reactogenicity and immunogenicity of the Takeda acellular pertussis vaccine combined with tetanus and diphtheria toxoids were compared in 139 infants aged 3 to 8 months, 60 infants and children aged 9 to 23 months, and 99 children aged 24 to 30 months. Good antibody responses to pertussis toxin (PT), filamentous hemagglutinin (FHA), and agglutinogens occurred in all age groups after both the third and fourth doses. After the fourth (booster) dose, the mean antibody values in initially seronegative infants vaccinated at 3 to 8 months of age were as follows: anti-PT, 67.

View Article and Find Full Text PDF

Serum zinc (Zn) and copper (Cu) concentrations were measured in infants weighing 740 g to 2,500 g at birth. They were divided into three groups depending on their birth weight; Group I ranged from 740 to 1,500 g (N = 35), Group II from 1,501 to 2,000 g (N = 26), and Group III from 2,001 to 2,500 g (N = 19). They were fed breast milk (N = 24), commercially available non-supplemented formula with low Zn and low Cu content (N = 42), or Zn- and Cu-supplemented formula (N = 14).

View Article and Find Full Text PDF

A sensitive enzyme immunoassay method (EIA) for an anticancer drug, aclacinomycin A (ACM), has been developed. With a double-antibody technique, ACM at a concentration as low as 100 pg/tube can be detected. An antibody to ACM was obtained by immunizing rabbits with an antigen prepared by coupling ACM with mercaptosuccinylated bovine serum albumin via N-maleoyl aminobutyric acid (MABA) as a coupling agent.

View Article and Find Full Text PDF

The specificity of antisera produced in rabbits for use in mitomycin C (MMC) enzyme immunoassay has been examined employing competitive experiments using several mitomycin analogs and the chemically or biologically degraded preparations of MMC. These studies demonstrate that the antiserum distinguished alterations in the chemical structure of the molecule, showing decreased immunoreactivity with mitomycin A (7.8%) and B (0.

View Article and Find Full Text PDF

A compact whole-body hyperthermia system utilizing extracorporeal circulation for anticancer treatment has been devised and its clinical applicability is discussed. Our system has the following advantages; 1. Our small heat-exchanger made of polyvinyl-chloride hollow fibers exhibited sufficient heat-exchanging capability.

View Article and Find Full Text PDF

A sensitive and specific enzyme immunoassay (EIA) for an anticancer drug, bleomycin (BLM), has been developed, which allows measurement of as little as 25 pg of the antibiotic/tube. An antibody to BLM was obtained by immunizing rabbits with an antigen prepared by conjugating BLM with mercaptosuccinylated bovine serum albumin via N-(gamma-maleimidobutyryloxy)succinimide as a coupling agent. Enzyme labeling of BLM was performed using beta-D-galactosidase (beta-Gal; EC 3.

View Article and Find Full Text PDF

An antibody specific for puromycin (PU) was prepared by immunization of rabbits with a PU conjugate of bovine serum albumin, which was newly synthesized by coupling PU to mercaptosuccinylated bovine serum albumin via a cross-linker, N-(gamma-maleimidobutyryloxy)succinimide. Enzyme labeling of PU was performed using beta-D-galactosidase [EC 3.2.

View Article and Find Full Text PDF

A mitomycin C (MMC) antibody was produced following immunization of rabbits with a MMC-bovine serum albumin conjugate, which was newly synthesized by coupling MMC to mercaptosuccinylated bovine serum albumin via a cross-linker, N-maleoyl aminobutyric acid. Enzyme labeling of MMC was performed using beta-D-galactosidase (EC 3.2.

View Article and Find Full Text PDF