Quantitative optical measurement of mitochondrial superoxide dynamics in pulmonary artery endothelial cells.

Reactive oxygen species (ROS) play a vital role in cell signaling and redox regulation, but when present in excess, lead to numerous pathologies. Detailed quantitative characterization of mitochondrial superoxide anion ( ) production in fetal pulmonary artery endothelia cells (PAECs) has never been reported. The aim of this study is to assess mitochondrial production in cultured PAECs over time using a novel quantitative optical approach.