Publications by authors named "Said A M Mazyad"

Ticks and blood samples were collected every month from March 2009 through April 2010 from different sites in Sinai to detect babesial parasites using PCR assay based on nuclear small subunit rRNA gene. Ticks were found to contain babesial DNA. Sequence determination and analysis of amplified portions of nss-rDNA revealed their identity with B.

View Article and Find Full Text PDF

A total of fifty stray dogs of both sex and of different ages were examined for natural infection with Echinococcus granulosus after post-mortem. Three main intestinal helminthes were recovered. These were in descending order of abundance Dipylidium caninum, Toxocara canis and E.

View Article and Find Full Text PDF

Antibodies against Coxiella burnetii were estimated among sheep, goats and camels (190), their owners (150 patients with pyrexia of unknown origin) and 30 normal individuals in North Sinai over the 2006 by indirect immounofluorescence assay. Nested polymerase chain reaction was used to detect Com-1 gene (genetic target of C. burnetii) encoding a 27-kDa outer membrane protein in the samples.

View Article and Find Full Text PDF

The effect of Juvenile hormone analogue, Admiral "pyriproxyfen", which acts as a protein de-naturing agent, on the protein patterns of D. farinae was studied on allergic reactions in patients with HDM asthma, and in mice injected twice (within 10 days) with mite crude extracts. Total protein concentrations and protein patterns were different in normal and admiral-treated mites.

View Article and Find Full Text PDF

Two insect growth regulators, Methorprene and Hydroprene were evaluated as growth hormones on the common house dust mite; Dermatophagoides farinae. The adults and immature stages were treated with 4 concentrations of each regulator as contact and as diet. The data were recorded 30 & 90 days post-exposure.

View Article and Find Full Text PDF

Laboratory study was conducted on the biology of the phorid fly Megaselia sacalaris. The influence of 3 constant temperatures and various larval densities was researched in the laboratory as these relate to mean life stage rate of development and survival. Flay development on a synthetic diet at 25 degrees C.

View Article and Find Full Text PDF

Three species of oribatid mites, Scheloribates zaherii, Zygoribatula tadrosi and Z. sayedi from pure colonies were experimentally exposed to infection by allowing them to feed on stool sheep infected with Moniezia expansa. The mites were followed up to the development of the infective cysticercoids.

View Article and Find Full Text PDF

Leishmania major promastigotes isolated from an Egyptian ZCL patient, was inoculated 4 x 10(6)/ml in a new culture medium, peptone-liver (P-L), with a final concentration of 10% of FCS (fetal calf serum), and incubated at 25+/-1 degrees C. Meanwhile, the same number of promastigotes were inoculated in Schneider's Drosophila medium as control. On the 10th day, number of L.

View Article and Find Full Text PDF

During the year 2001, a total of 475 sheep, 200 goats, 135 cattle and 190 camels in El Arich city and El Hassanah center were examined for Babesia ovis and Theileria ovis. Blood films were taken from the vein of the ear. Meanwhile, the animals were examined for tick infestations.

View Article and Find Full Text PDF

Examination of sheep and goats in four areas in North Sinai revealed an overall infection of 12.70% with Fasciola species, 11.8% with Paramphistomum cerve, 12.

View Article and Find Full Text PDF