Publications by authors named "Sai Fan"
Ovalbumin (OVA) is a high-risk allergen with complex tertiary structure in food samples. Here, we developed an accurate UPLC-MS/MS-based assay to improve OVA quantitative performance in processed foods. Full-length isotope-labeled OVA proteins (OVA-I) were synthesized using stable isotope labeling by amino acids in cell culture (SILAC) technique and employed as functional internal standards to ensure similar cleavage sites between internal standards and analytes.
View Article and Find Full Text PDFOrganophosphate esters (OPEs) and their metabolites (mOPEs) are emerging pollutants. In this study, 18 OPEs and 10 mOPEs were measured in the 6th and 7th Beijing total diet studies (TDSs), and the dietary intakes of these pollutants by Beijing adults were estimated to assess related health concerns. Most OPEs and mOPEs had high detecting frequencies in both TDSs, which indicated that various foods in Beijing have been universally contaminated with OPEs and mOPEs.
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- Organophosphate esters (OPEs) and their metabolites (mOPEs) are increasingly recognized as harmful environmental pollutants, prompting the need for better analytical methods to measure them in human samples.
- This study developed a streamlined analytical technique using cold-induced liquid-liquid extraction (CI-LLE) and HPLC-MS/MS to simultaneously detect 18 OPEs and 10 mOPEs in human serum, urine, and milk.
- Results confirmed that the method had high sensitivity (with detection limits as low as 0.1 pg/mL), good recovery rates (80-123%), and minimal interference from the sample matrix, highlighting the widespread presence of OPEs and mOPEs in humans.
Objective: To investigate the pollution of organophosphate esters(OPEs) and their metabolites in drinking water in Dongcheng District of Beijing, and to assess the exposure risk of adults in drinking water.
Methods: The contents of 14 OPEs and 7 metabolites in drinking water were determined by automatic solid phase extraction, isotope dilution and liquid chromatography tandem mass spectrometry. The average daily potential dose(ADD) were calculated based on the recommended intake of drinking water.
Article Synopsis
- The study aimed to develop an efficient method for detecting 21 organophosphate esters and their metabolites in drinking water using automatic solid phase extraction followed by liquid chromatography-tandem mass spectrometry.
- The method exhibited high sensitivity with detection limits between 0.01-0.24 ng/L and good recovery rates of 57.6%-121.2%, demonstrating its effectiveness when applied to various water samples, including tap and packaged water.
- Overall, the approach is characterized as simple and suitable for high-throughput analysis, highlighting potential risks associated with organophosphate exposure in drinking water.
Objective: To develop a method for rapid determination of Bacillus cereus cereulide in rice and flour products by ultra-performance liquid chromatography-tandem mass spectrometry, providing emergency measures for food poisoning caused by cereulide.
Methods: Single rice and flour samples were extracted with acetonitrile aqueous solution, salting out, after centrifuged and filmed, the organic phase was directly determined. The complex matrix samples fried rice and noodles were extracted with acetonitrile aqueous solution, cleaned up with HLB column, a ACQUITY UPLC Peptide BEH C_(18) 300Å column(2.
Article Synopsis
- The study investigated the impact of multiple endocrine disruptors (EDCs) on thyroid hormones in 177 healthy adults from China, measuring both serum thyroid hormone levels and urinary EDCs.
- Results showed that, after accounting for various factors, bisphenol F (BPF) significantly correlated with lower levels of several thyroid hormones, while certain phthalate metabolites were positively linked to thyroid hormones.
- The researchers highlighted a dose-response relationship between the mixture of EDCs and thyroid hormone levels, particularly emphasizing the strong influence of BPF on thyroid function, suggesting that even low-level EDC exposure could disrupt hormonal balance in healthy adults.
Article Synopsis
- The global incidence of thyroid cancer, particularly papillary thyroid cancer (PTC), has significantly risen, with some endocrine disruptors possibly linked to cancer processes, though specific relationships with bisphenol analogs remain uncertain.
- A case-control study investigated the relationship between urinary concentrations of bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS) and the risk of PTC, finding a positive association with BPF while BPA and BPS showed negative associations.
- The results indicated that BPF exposure increases PTC risk, notably in non-smoking women, warranting further research to confirm these findings.
Objective: A method for the rapid determination of dichlorvos, trichlorfon, fenthion, fenthion-sulfone, fenthion-sulfoxide, fenthion-oxon, fenthion-oxon-sulfone, fenthion-oxon-sulfoxide, phoxim, propetamphos, malathion, diazinon and coumaphos 13 common organophosphorus pesticides and their metabolites poison residues in milk of cows and sheep by ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with passing type solid phase extraction(SPE) purification was developed.
Methods: After centrifugation at 4 ℃, the milk was purified by passing type SPE with acetonitrile precipitating protein and determined by UPLC-MS/MS in electrospray positive ion mode(ESI+) and multi-reaction monitoring scanning(MRM), external standard method for quantitative analysis with matrix matching standard curve.
Results: The recoveries of 13 target compounds were between 81.
Organophosphate esters (OPEs) and their diester metabolites have been frequently found in various environmental matrices and regarded as emerging environmental pollutants, whereas data on their occurrence in foods and human matrices are still limited. In this study, a novel and simple procedure was developed to simultaneously determine 14 OPEs and 6 diester metabolites in dairy products and human milk. After enzymatic hydrolysis by β-glucuronidase/arylsulfatase, a freeze-dried milk sample was extracted with acetonitrile and purified by solid-phase extraction.
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- Organophosphate esters (OPEs) and their metabolites (di-OPEs) are common in the environment and were measured in infant formula and baby foods in Beijing, showing high detection rates.
- The study found varying concentrations of OPEs, with triphenyl phosphate (TPhP) being the most common in infant formulas and tri(3-chloropropyl) phosphate (TCIPP) in baby supplementary foods.
- Despite OPE levels being higher in infants than in adults, the estimated dietary intakes of OPEs via these foods do not appear to pose significant health risks for infants.
Although diet is regarded as a major exposure source of organophosphate esters (OPEs), the dietary survey of OPEs in China has been limited. Based on the sixth Chinese Total Diet Study (TDS) conducted during 2016-2019 in 24 of 34 provinces in China, 14 OPEs were detected in 96 food composites from four animal-origin food categories. Twelve OPEs were detected in more than 80% of the samples and 2-ethylhexyl diphenyl phosphate (EHDPP) presented the highest median concentration (1.
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- A reliable headspace gas chromatography-mass spectrometry (HS-GC-MS) method was developed to analyze furan levels in canned foods and packaged beverages.
- The method uses D_4-furan as an internal standard, with a linear detection range of 2.0-200.0 ng and a low quantification limit of 1.0 ng, showing high accuracy and reproducibility.
- Testing 59 samples revealed widespread furan contamination, especially in products like coffee and milk tea, with some samples containing up to 153.99 ng of furan.
Organophosphorus flame retardants are a class of widely used plasticizers and flame retardants. In this study, an analytical methodology for the simultaneous determination of 13 organophosphorus flame retardants in milk was developed by using high-performance liquid chromatography-tandem mass spectrometry in combination with a modified quick, easy, cheap, effective, rugged, and safe technique and solid-phase extraction. The experimental parameters of the sample purification procedure and high-performance liquid chromatography-tandem mass spectrometry were optimized.
View Article and Find Full Text PDFDietary exposure of general Chinese population to fatty acid esters of 3-monochloropropane-1, 2-diol (3-MCPD) from edible oils and oil-containing foods.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2021
Article Synopsis
- Edible oils and oil-containing foods are primary sources of 3-monochloropropane-1,2-diol (3-MCPD) esters, prompting analysis of 3,847 food samples from various regions in China between 2015-2017.
- The study found the mean levels of 3-MCPD esters in edible oils, fried foods, and bakery items to be relatively low, with daily intake estimates generally below the tolerable limits set by the European Food Safety Authority.
- While most of the Chinese population faces low health risks from 3-MCPD esters, individuals with high oil consumption may exceed safety thresholds, requiring further attention to their dietary habits.
Objective: To establish an analytical method for determination of 20 kinds of β-receptor blockers residues in animal foods by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
Methods: The samples of animal foods were enzymatic hydrolysis by trichloroacetic acid(TCA), purified by MCX column. The separation was performed on a Waters ACQUITYTM BEH C_(18 )column(100 mm×2.
Objective: To establish an analytical method for determination of 6 kinds of α_2-agonists in animal foods by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
Methods: The samples of animal food were enzymatic hydrolysis by β-glucosidase/arylsulfatase, purified by MCX column. The separation was performed on a Dikma leapsil C_(18) column(2.
Objective: To establish a method for simultaneous determination of L-carnitine, choline, taurin in infant by ultrafiltration tube cleaning and ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
Methods: The sample was dissolved in water and acid hydrolyzed, then adjusted pH with a sodium hydroxide solution. After ultrafiltration tube cleaning and centrifugation, the sample solution was separated on an amide column ACQUITY UPLC BEH Amide(2.
Objective: To establish a method for determination of ochratoxin A and ochratoxin alpha in wine by ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) based on isotopic internal standard method.
Methods: The wine sample was adjusted to pH 9. 0 by 5% ammonia and concentrated by a MAX solid phase extraction cartridge.
Objective: A method for the simultaneous determination of 5 kinds of fish anesthetics residues in fish has been developed by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Eugenol, methyl-eugenol, methyl-isoeugenol, acetyl-isoeugenol and tricaine methanesulfonate(MS-222) were concerned.
Methods: After homogenization fish samples were extracted by acetonitrile-water(80↿0, V/V), purified by Oasis PRiME HLB solid-phase extraction column.
Acyl coenzyme A (CoA)-binding protein (ACBP) can bind acyl-CoAs with high specificity and affinity, thus playing multiple roles in cellular functions. Mitochondria of the apicomplexan parasite have emerged as key organelles for lipid metabolism and signaling transduction. However, the rationale for how this parasite utilizes acyl-CoA-binding protein to regulate mitochondrial lipid metabolism remains unclear.
View Article and Find Full Text PDFBackground: The incidence of thyroid cancer has recently increased worldwide. With the exception of radiation exposure, the effects of potential risk factors on thyroid cancer incidence remain controversial.
Objectives: The association between exposure to iodine, perchlorate, and thiocyanate and papillary thyroid cancer (PTC) incidence was evaluated and risk factors were predicted.
Objective: A method for the determination of 6-benzylaminopurine( 6-BAP), isopentennyladenine( z-IP), 4-fluorophenoxyacetic acid( 4-FPA), 4-chlorophenoxyacetic acid( 4-CPA) in bean sprout was developed using solid phase extraction column with ultra-high performance liquid chromatography.
Methods: The sample was extracted by acetonitrile,dehydrated by salt,then centrifugation,and purified by PXC/PWA solid phase extraction column. The chromatographic analysis was carried out on C18 chromatographic column( 100 mm ×2.
Objective: A method for determination of phthalate esters and their metabolites in blood by using SPE-ultra performance liquid chromatography tandem mass spectrometry.
Methods: A stable isotope detection method been developed for the determination of 20 kinds of phthalate esters and 6 kinds of phthalate esters metabolites in blood by ultra-performance liquid chromatography-mass spectrometry( UPLC-MS) coupled with SPE column purification. Blood samples were diluted with acetate buffer( pH5.
Objective: To develop a method for determination of 8 veterinary drug residues in chicken matrix by Qu ECh ERS-ultra performance liquid chromatography tandem mass spectrometry.
Methods: Chicken samples were extracted with acid acetonitrile, salting out, and then the organic phase were cleaned up by C18 and PSA. A Waters ACQUITYTM BEH C18column( 100 mm × 2.