Cytosolic peptide:N-glycanase (cPNGase) is an enzyme found in eukaryotic cells that helps remove sugar molecules from misfolded proteins as part of cellular quality control.
The study focused on demonstrating the activity of cPNGase in plants, specifically using a crude extract from a genetically modified Arabidopsis thaliana that lacks two specific PNGase genes.
The research provides direct evidence of cPNGase's ability to act on a denatured glycoprotein in plants, enhancing our understanding of protein quality control mechanisms.