Comparative study of demineralized collagen degradation determined by hydroxyproline assay and microscopic depth measurement.

Introduction: Quantification of collagen degradation is an important parameter to evaluate dentin caries progression or the efficacy of caries prevention aid. The aim of this study was to validate the simple light microscopic technique (LM) to evaluate collagen degradation by comparing with hydroxyproline assay technique (HPN).

Materials And Methods: Bovine root dentin blocks were embedded in acrylic resin, polished and covered with nail varnish except a 1.

Formation and characterization of hypermineralized zone beneath dentine lesion body induced by topical fluoride in-vitro.

Objective: This in-vitro study aimed to evaluate and characterize the hypermineralized zone (Hyper-zone) formed beneath the remineralized dentine lesion body by transverse microradiography (TMR), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDS).

Design: Demineralized bovine dentine specimens were treated with fluoride solutions (APF, NaF) and remineralized for 2-4 weeks. Then thin sections were prepared to characterize the Hyper-zone by TMR, EDS.