Controlled nanostructrures formation by ultra fast laser pulses for color marking.

Precise nanostructuration of surface and the subsequent upgrades in material properties is a strong outcome of ultra fast laser irradiations. Material characteristics can be designed on mesoscopic scales, carrying new optical properties. We demonstrate in this work, the possibility of achieving material modifications using ultra short pulses, via polarization dependent structures generation, that can generate specific color patterns.

[Examination over a foreign body left inside during an operation of a lumbar spine].

Procedure of an examination over a gauze swab removed during a repeated operation of a patient who had undergone earlier a neurosurgical operation of a lumbar spine has been described. The extracted gauze swab became an exhibit in a case at court for financial compensation. The court commissioned to explain "if it could be placed in another way than leaving it during the former surgical operation".

[VNTR-PCR in diagnosis of inherited Rb gene mutation].

Molecular genetic analysis of DNA by PCR-VNTR was performed in a family with hereditary retinoblastoma presenting some difficulties in pedigree analysis. PCR-VNTR allowed to perform a more accurate counselling in this family. Analysis of the way of transmission, carrier status exclusion (in two persons) and confirmation (in one) was possible only by VNTR-PCR.

[RFLP-PCR technique for exclusion of carrier status of the mutated Rb gene].

Molecular-genetic analysis of DNA by PCR-RFLP technique in a family with a case of unilateral retinoblastoma was performed. The carrier status of mutated Rb gene in a sister of proband was excluded on the basis of molecular analysis despite unsynonymous pedigree data.

Failure to detect antihuman-immunoglobulinallotype-active antibodies in diagnostic sera directed against some enterobacteria.

Diagnostic sera to determine antigenic properties of bacteria were tested to clarify the question whether these sera also contain antibodies being active against human immunoglobulin allotypes. Sera directed against various strains of Escherichia coli, Salmonella, and Shigella were found to be negative for anti-Ig-allotype activity.

[Possible common partial antigens in human Ig allotype structure and ubiquitous bacteria, studied with the example of Escherichia coli].

As can be learned from the literature, bovine serum may contain antibodies directed against human immunoglobulin allotypes. This gave rise to the question of what the origin of those antibodies is. We tested bacteria (E.

Isoelectric focusing of the human IgG light chains: a classification of the fractions and their normal values.

The method of isoelectrofocusing was employed to obtain a reproducible pattern of human IgG light chain bands. The map of IF spectra, which contained 13 main fractions and some subfractions, was delineated. Isoelectric points of these fractions and their quantitative relations in 10 healthy individuals were determined.

Existence of the natural antibody against human-gamma-globulin in bovine sera.

The natural antibody against human gamma-globulins has been found in 2 out of 26 bovine sera. The bovine sera were absorbed with human erythrocytes of group O and tested with human red cells coated with incomplete antibodies. Two of 26 sera studied displayed evident agglutinating properties similar to those of anti-human globulin used in Coombs test.

INBORN ENZYMATIC DEFECT AS THE PROBABLE CAUSE OF THE FORMATION OF RENAL STONES CONSISTING OF URIC ACID.

In four persons of one family the existence of an enzymatic defect, presumably consisting of a deficiency of glutaminase in the cells of the renal tubules, is postulated, and is implied by a reduced elimination of ammonia in the urine, by a relatively low urinary pH, and by its increased titratable acidity. The most characteristic clinical symptom is irritation of the distal part of the urinary tract, connected with numerous crystals of uric acid appearing in the urinary sediment. The elimination of uric acid is normal, or even reduced, and the level of uric acid in the blood serum is also within normal limits.