Parasite-induced IFN-γ regulates host defense via CD115 and mTOR-dependent mechanism of tissue-resident macrophage death.

Host resistance to a common protozoan parasite Toxoplasma gondii relies on a coordinated immune response involving multiple cell types, including macrophages. Embryonically seeded tissue-resident macrophages (TRMs) play a critical role in maintaining tissue homeostasis, but their role in parasite clearance is poorly understood. In this study, we uncovered a crucial aspect of host defense against T.

[Аnalysis of global trends in the use of whole-grain products in the nutrition of the population].

In recent years, there was a worldwide increase of interest in foods with whole grain components. The number of studies that confirm their wide functional potential and importance in the prevention of chronic non-communicable diseases is expanding. At the same time, there is no agreement reached on the definition of the concept of whole grain products and the optimal level of its consumption in the world; and the provisions enshrined in national regulations are quite contradictory.

Combined System for the Simultaneous Delivery of Levofloxacin and Rifampicin: Structural and Functional Properties and Antibacterial Activity.

The therapy of resistant forms of tuberculosis requires the simultaneous use of several drugs, in particular, a combination of rifampicin and levofloxacin. In this paper, we aimed to design a combined system for the simultaneous delivery of these drugs for potential inhalation administration. A feature of this system is the incorporation of rifampicin into optimized liposomal vesicles capable of forming a multipoint non-covalent complex with chitosan-β-cyclodextrin conjugates.

Liposomal Forms of Fluoroquinolones and Antifibrotics Decorated with Mannosylated Chitosan for Inhalation Drug Delivery.

The severe course of COVID-19 leads to the long-terming pulmonary diseases, such as bacterial pneumonia and post-COVID-19 pulmonary fibrosis. Thus, the essential task of biomedicine is a design of new effective drug formulations, including those for inhalation administration. In this work, we propose an approach to the creation of lipid-polymer delivery systems for fluoroquinolones and pirfenidone based on liposomes of various compositions decorated with mucoadhesive mannosylated chitosan.

Sulfonamide drugs: Low-cost spectrofluorometric determination using a computer monitor calibrator for detection.

A possibility of the use of a common monitor calibrator as a portable and inexpensive tool for the fluorometric determination of sulfonamide drugs after their reaction with fluorescamine was examined. The luminescence measurements with a calibrator are based on irradiation of a test sample by the device lamp with a broadband spectrum in the visible and near UV regions and simultaneous registration of the secondary radiation by the device detector. Two types of cuvettes with black light absorbing sides eliminating the reflected self-radiation were tested.

[Role of Kraevsky cells in forensic histological diagnostics].

The study objective is to establish the role of light hepatocytes (Kraevsky cells) for forensic histological diagnosis of glycogen depletion in the liver due to various conditions, including shock. A standard histological examination of liver samples of persons who died from various causes was performed; routine staining with hematoxylin and eosin and additional staining by the PAS method was used. Histotopographic comparison of cross sections was performed to clarify the localization of light hepatocytes with different PAS staining results.

Choosing the optimal mandible position for inferior alveolar nerve block (IANB) using finite element analysis.

Background: One of the most popular methods of local anesthesia in dentistry, inferior alveolar nerve block (IANB) involves the blockade of the inferior alveolar nerve (IAN) and lingual nerve (LN) in the pterygomandibular space. Despite the large number of works describing the contents of this space, the spatial displacements of the anatomical structures of this area at different positions of the mandible have not been sufficiently studied. The aim of our study was to study the spatial movements of the IAN and inferior alveolar artery (IAA) at various positions of the mandible using computer simulation and finite element analysis to find the safest way to conduct IANB.

The Influence of Lipid Matrix Composition on the Microenvironment of Levofloxacin in Liposomal Forms.

We have studied the interaction of the antibacterial drug levofloxacin with lipid bilayers of various compositions: 100% DPPC and with the addition of 20% cardiolipin. For DPPC liposomes, levofloxacin was found to penetrate into the subpolar region at the lipid-water interface. The role of the anionic lipid in the interaction of an active molecule with a bilayer has been established: levofloxacin enters the microenvironment of the phosphate group, displacing water, and does not penetrate into the hydrophobic part of the bilayer.

IFN-γ mediates Paneth cell death via suppression of mTOR.

Paneth cells constitutively produce antimicrobial peptides and growth factors that allow for intestinal homeostasis, host protection, and intestinal stem cell replication. Paneth cells rely heavily on the glycolytic metabolic program, which is in part controlled by the kinase complex Mechanistic target of rapamycin (mTORC1). Yet, little is known about mTOR importance in Paneth cell integrity under steady-state and inflammatory conditions.

[The development of children nutrition science in Russia].

The article presents the main directions of children nutrition science in Russia, that starts at the Institute of Nutrition (nowadays Federal Research Centre of Nutrition, Biotechnology and Food Safety). Almost all areas of children nutrition - from early age to adulthood, from the organization of public nutrition to personalized approaches - were developed on the base of the Department of Children Nutrition, founded in 1932. Years of beneficial work of famous Russian specialists in the field of pediatric dietetics and nutrition science are associated with this Department, for instance professors E.

Future transport policy designs for biomethane promotion: A system Dynamics model.

In this paper, we present a dynamic simulation tool for biomethane policy design in Latvia. A new System Dynamics based computer simulation model covering the whole biogas-to-biomethane value chain including bioresource availability, biogas production and biogas utilization pathways is proposed. The goal of the model is to evaluate the efficiency of a set of policy instruments on increasing the transition from biogas for electricity production to biogas upgrading to be used as a transportation fuel thus contributing to national renewable energy targets in the transport sector by 2030.

Moxifloxacin interacts with lipid bilayer, causing dramatic changes in its structure and phase transitions.

Most drugs besides their intended activity, express undesired side effects, including those with the engagement of cell membrane. Previously, such undesired nonspecific effects on the membrane have been shown for a number of widely used nonsteroidal anti-inflammatory drugs. In this paper, we study the mechanism of interaction between moxifloxacin (Mox), antibacterial drug of broad specificity, with lipid bilayer of the liposomes of various compositions as a model of cell membrane using a combination of spectroscopy methods, including ATR-FTIR spectroscopy, circular dichroism, UV and fluorescence spectroscopy.

[The role of dietary fibers in the nutrition of the population].

Dietary fibers are the important components of the diet with functional properties. The importance of optimal intake of dietary fibers for saving health, gastrointestinal functions, prevention of metabolic disorders (overweight, obesity, hyperlipidemia), reducing the risk of cardiovascular and cancer diseases has been proved. The dose-dependent effect of dietary fibers, their quantitative and qualitative characteristics, as well as food sources in the structure of nutrition are discussed.

Alarmin S100A11 initiates a chemokine response to the human pathogen Toxoplasma gondii.

Toxoplasma gondii is a common protozoan parasite that infects up to one third of the world's population. Notably, very little is known about innate immune sensing mechanisms for this obligate intracellular parasite by human cells. Here, by applying an unbiased biochemical screening approach, we show that human monocytes recognized the presence of T.

A New Method and Mass-Spectrometric Instrument for Extraterrestrial Microbial Life Detection Using the Elemental Composition Analyses of Martian Regolith and Permafrost/Ice.

We propose a new technique for the detection of microorganisms by elemental composition analyses of a sample extracted from regolith, permafrost, and ice of extraterrestrial bodies. We also describe the design of the ABIMAS instrument, which consists of the onboard time-of-flight laser mass-reflectron (TOF LMR) and the sample preparation unit (SPU) for biomass extraction. This instrument was initially approved to fly on board the ExoMars 2020 lander mission.

[The study of rs11801197 polymorphism of the calcitonin receptor gene () in Moscow women and children with different level of bone strength].

The frequency of the polymorphism rs11801197 of calcitonin receptor gene (CALCR) was studied by real-time PCR in 422 Moscow women and children, including pregnant women (n=96), lactating (n=29) and non-pregnant women (n=28) and children (n=269) of preschool (2-6 years, n=76) and school age (7-16 years, n=193) with different levels of bone strength (BS) as determined by ultrasound densitometry. It was found that the decrease in the value of the BS (Z-score<-1) was observed in 60% of women, 54% of preschool children and 48% of school children. In the cohort studied the predominant genotype of rs11801197 polymorphism of CALCR gene was CT (38%), the frequency of the genotypes CC and TT - 31%, C and T allele - 50%.

Influence of Xe and Kr impurities on x-ray yield from debris-free plasma x-ray sources with an Ar supersonic gas jet irradiated by femtosecond near-infrared-wavelength laser pulses.

Many aspects of physical phenomena occurring when an intense laser pulse with subpicosecond duration and an intensity of 10^{18}-10^{19}W/cm^{2} heats an underdense plasma in a supersonic clustered gas jet are studied to determine the relative contribution of thermal and nonthermal processes to soft- and hard-x-ray emission from debris-free plasmas. Experiments were performed at the University of Nevada, Reno (UNR) Leopard laser operated with a 15-J, 350-fs pulse and different pulse contrasts (10^{7} or 10^{5}). The supersonic linear (elongated) nozzle generated Xe cluster-monomer gas jets as well as jets with Kr-Ar or Xe-Kr-Ar mixtures with densities of 10^{18}-10^{19}cm^{-3}.

L-shell spectroscopic diagnostics of radiation from krypton HED plasma sources.

X-ray spectroscopy is a useful tool for diagnosing plasma sources due to its non-invasive nature. One such source is the dense plasma focus (DPF). Recent interest has developed to demonstrate its potential application as a soft x-ray source.

[Sufficiency with water-soluble vitamins and state of bone in pregnant women].

Vitamin status and bone strength have been estimated in 91 pregnant women (29.3 ± 4.6 years old) from Moscow by non-invasive methods.

[The study of rs1800012 polymorphism of the alphal-chain collagen type 1 gene in Moscow women and children with different level of bone strength].

The frequency of rs1800012 polymorphism of alpha1-chains of type 1 collagen gene (COL1A1) was studied by real-time PCR in 422 Moscow women and children, including pregnant women (n=96), lactating (n=29) and non-pregnant women (n=28) and preschool children (2-6 years, n=76) and school children (7-16 years, n=193) with different levels of bone strength (BS) as determined by ultrasound densitometry. It was found that the decrease in the value of the BS (Z-score < -1) was observed in 60% of women, 54% of preschool children and 48% of school-aged children. In the surveyed cohort the predominant genotype of rs1800012 polymorphism CO1A1 geneI gene was GG (55.

[The prevalence of overweight and obesity in moscow pregnant women and principles of alimentary correction of these conditions].

The aim was to study the prevalence of overweight and obesity in Moscow pregnant women, as well as to conduct a comparative study of normal and excessive women`s body weight influence on the course and outcome of pregnancy and delivery, anthropometric indices and state of newborn`s health, to assess women's dietary intake and to develop approaches to nutritional correction of overweight and obesity in pregnant women. The prevalence of overweight and obesity among pregnant women in Moscow in 2009-2011 was 8.3% of the total number of pregnant women observed.

Compact hohlraum configuration with parallel planar-wire-array x-ray sources at the 1.7-MA Zebra generator.

A compact Z-pinch x-ray hohlraum design with parallel-driven x-ray sources is experimentally demonstrated in a configuration with a central target and tailored shine shields at a 1.7-MA Zebra generator. Driving in parallel two magnetically decoupled compact double-planar-wire Z pinches has demonstrated the generation of synchronized x-ray bursts that correlated well in time with x-ray emission from a central reemission target.

Growth and Tolerance Assessment of a Lutein-fortified Infant Formula.

Purpose: To evaluate safety, gastrointestinal tolerance, and growth of a new experimental starter formula (NESF) fortified with lutein, prebiotics, probiotics, nucleotides and beta-carotene, fed to infants within the first months of life.

Methods: This was a non-randomized, open, uncontrolled study conducted from December 2010 to May 2011. Twenty-three healthy infants aged 10 days to 2 months old were enrolled.

[Bone mineral density in pregnant women from Moscow: possible effects of pregnancy dynamics and nutrient intake].

Supporting of bone health is one of the main approaches to provide health in pregnant women considering intensive calcium and other mineral mobilization from mass bone that is necessary forforming fetus bone. This mobilization may lead to decrease of bone mineral density and development of osteopenia and osteoporosis. The important factors of development of bone impairment in pregnancy are nutrition and particular deficient consumption of protein, Ca, vitamin D.

Wire ablation dynamics model and its application to imploding wire arrays of different geometries.

The paper presents an extended description of the amplified wire ablation dynamics model (WADM), which accounts in a single simulation for the processes of wire ablation and implosion of a wire array load of arbitrary geometry and wire material composition. To investigate the role of wire ablation effects, the implosions of cylindrical and planar wire array loads at the university based generators Cobra (Cornell University) and Zebra (University of Nevada, Reno) have been analyzed. The analysis of the experimental data shows that the wire mass ablation rate can be described as a function of the current through the wire and some coefficient defined by the wire material properties.