[Cell technologies as a basis for the development of regenerative principles for the treatment of lacrimal gland diseases].

The lacrimal gland (LG) is a tubuloacinar exocrine gland composed of acinar, ductal, and myoepithelial cells. Three-dimensional distribution of acinar lobules, ducts, and myoepithelial cells is necessary for the effective functioning of the organ. LG is the main organ of immune surveillance of the ocular surface system.

Role of phosphate-coordinating arginine residues in the thermal stability of uridine phosphorylase from Shewanella oneidensis MR-1.

The role of phosphate-coordinating arginine residues in the thermal stability of uridine phosphorylase from Shewanella oneidensis MR-1 was investigated by mutation analysis. Uridine phosphorylase mutant genes were constructed by site-directed mutagenesis. The enzyme mutants were prepared and isolated, and their kinetic parameters were determined.

[Predictive significance of genetic analysis of the development of dry eye disease of different origin].

Unlabelled: One of the etiological causes of dry eye disease (DED) is systemic autoimmune diseases (AID): primary Sjögren's syndrome (PSS), rheumatoid arthritis (RA); their manifestation may begin with ophthalmic symptoms. The relationship of PSS and RA with genetic factors is proven. The contribution of polymorphic markers of the genes , , , , in the development of these diseases is established, as well as their connection with the development of DED.

[Neuropathic pain in dry eye syndrome. Part 2. Clinical picture, diagnosis and treatment].

Burning eye syndrome is a chronic neuropathic pain syndrome, which is characterized by dysesthesia, spontaneous pain, allodynia and hyperalgesia. The review describes clinical features and presents available data on possible methods of diagnosis and therapy of this condition.

[Neuropathic pain in dry eye syndrome. Part 1. Pathophysiological mechanisms of pain formation].

The review details the features and mechanisms of the formation of various types of pain. The emphasis is placed on the occurrence of pain syndrome in various ophthalmological diseases, particularly in dry eye syndrome. The article also presents literature data on the role of cytokines in the formation of a neuroinflammatory cascade affecting damage to corneal nerve fibers and the development of pain syndrome, which is a characteristic feature of a subtype of dry eye disease - burning eye syndrome.

[The role of miRNA in the pathogenesis of diseases associated with functional dysregulation of the lacrimal gland].

At this time, the mechanism causing lacrimal gland dysfunction is not understood completely. In diseases associated with lacrimal gland involvement (Sjogren's syndrome, sarcoidosis, IgG4-associated disease, etc.) patients have been observed to experience elevated cellular apoptosis, active production of autoantibodies to glandular tissue, increased level of pro-inflammatory cytokines, functional disruption of signaling molecules leading to changes in tear production.

[Evolution of dry eye disease diagnostics].

This article reviews modern functional and instrumental examination methods included in the diagnostic algorithm for dry eye disease. The described methods can serve as an objective criterion for the effectiveness of the therapy.

[Features of water-electrolyte component of the tear fluid].

Tear production is a complex multi-step process that can be arbitrarily divided into three stages: «primary» secretion by the acinar cells of the main lacrimal glands, formation of «secondary» lacrimal fluid in the ducts of the main lacrimal glands, and «tertiary» modification of the tear composition in the conjunctival sac. This article highlights mechanisms of water and electrolytes secretion in the process of tear fluid production and describes the particularities of distribution of the membrane transport proteins in the lacrimal gland and the ocular surface.

[Modern possibilities of studying the composition of meibomian glands secretion].

As the main source of various lipids, the meibomian glands are involved in the formation of lipid layer of the tear film and the maintenance of homeostasis of the ocular surface. This process is directly dependent on the chemical composition and thickness of the lipid layer. In addition to lipid components, the meibum also contains various proteins that affect the properties of the tear film.

Vanadate as a new substrate for nucleoside phosphorylases.

Orthovanadate was shown to serve as a substrate for nucleoside phosphorylases from Escherichia coli, Shewanella oneidensis, Geobacillus stearothermophilus, and Halomonas chromatireducens AGD 8-3. An exception is thymidine phosphorylase from the extremophilic haloalkaliphilic bacterium Halomonas chromatireducens AGD 8-3, which cannot catalyze the vanadolysis of nucleosides. The kinetic parameters of nucleoside vanadolysis were evaluated.

[Association of polymorphic markers rs7947461 of the TRIM21 gene and rs33996649 of the PTPN22 gene with the risk of developing exogenous dry eye syndrome].

Unlabelled: In this age of technological advancement, an increasing number of people is being exposed to external risk factors of damaging their ocular surface (wearing contact lenses, electromagnetic radiation from computers, mobile devices, etc.). However, the presence of external factors does not lead to a 100% risk of developing the dry eye disease (DED).

Inactivation of Native K Channels.

We have experimented with isolated cardiomyocytes of mollusks Helix. During the whole-cell patch-clamp recordings of K currents a considerable decrease in amplitude was observed upon repeated voltage steps at 0.96 Hz.

[Experimental investigation of the safety of terahertz radiation in corneal hydration assessment].

Unlabelled: Application of terahertz (THz) radiation in novel non-invasive biomedical technologies has recently received considerable attention. However, experimental data about the safety of exposure to THz radiation for biological objects (including eye structures ) are limited. To our knowledge, the safety of THz reflectometry (frequency range of 0.

[The relationship between Sjogren's syndrome, systemic sclerosis and lymphoproliferative diseases].

Unlabelled: Despite the large number of studies devoted to the study of systemic sclerosis (SSc), the high risk of developing lymphomas in this disease, the relationship of their development with certain subtypes of SSc and specific SSc-associated autoantibodies is still debated in the literature.

Aim: To study demographic, clinical, laboratory and immunological characteristics of patients with a combination of primary Sjogrens syndrome (pSS) and SSc and diagnosed lymphoproliferative diseases (LPDs); to characterize morphological/immunomorphological variants and course of non-Hodgkins lymphomas (NHL), developing in patients with these rheumatic diseases (RDs).

Materials And Methods: In 19982018 at the Nasonova Research Institute of Rheumatology, 13 patients with clinical and laboratory manifestations of pSS (12) and SSc (13) were diagnosed with various lymphoproliferative diseases (LPDs).

[Morphofunctional substantiation of repeated invasive treatment of chronic blepharitis].

Unlabelled: The expanding range of diagnostic instrumental methods allows an in-depth study of the morphological and functional state of the eyelids, which is the basis for determining the strategy for the treatment of chronic blepharitis and subsequent timely supplementation and altering of its algorithm.

Purpose: To substantiate the repeated courses of invasive treatment of chronic blepharitis based on morphological and functional studies.

Material And Methods: The study included 45 patients (90 eyes) with chronic mixed blepharitis.

The Role of Polymorphic Markers rs1478604, rs2292305, and rs2228262 in THBS1 Gene in the Development of Autoimmune Dry Eye Syndrome.

An association of polymorphic marker rs2228262 in the THBS1 gene with the risk of developing dry eye in Sjögren syndrome was revealed. Confocal microscopy data suggest that this polymorphic marker is responsible for the high probability of corneal nerve fiber lesion in Sjögren syndrome even in the absence of clinical and functional signs of dry eye syndrome. A significant correlation was established between polymorphic markers rs1478604, rs2228262 in THBS1 gene and the coefficients of anisotropy and orientation symmetry of corneal nerve fibers.

[Clinical and laboratory characteristics of IgG4-realated disease and its diagnostic algorithm].

Aim: to propose diagnostic algorithm of IgG4-related disease (IgG4-RD).

Materials And Methods: One center retrospective research. 52 pts with IgG4-RD were included.

[Treatment of chronic posterior blepharitis after allogeneic hematopoietic stem cell transplantation (a case report)].

After allogeneic hematopoietic stem cell transplantation (allo-HSCT), various eye diseases are detected in 30-60% of patients, with chronic graft-versus-host reaction - in 50-90% of patients. Among the complications, the most frequent is damage to the eye surface and eyelids. The article presents a clinical case of successful minimally invasive treatment of chronic posterior blepharitis in a patient who has undergone allogeneic hematopoietic stem cell transplantation.

[Treatment of chronic blepharitis].

The most effective method of treating chronic blepharitis - a disease with multifactorial pathogenesis requiring an individual approach - is a combination of remedial measures that addresses eyelids hygiene and includes medicated and device therapy.

[Effectiveness of a nonsteroidal anti-inflammatory drug in the treatment of chronic non-infectious blepharitis].

Unlabelled: Anti-inflammatory therapy is a type of etiopathogenetic treatment of chronic mixed blepharitis. Nonsteroidal anti-inflammatory drugs (NSAIDs) have pronounced anti-inflammatory effect comparable to the action of corticosteroids.

Purpose: To assess of the effectiveness of a NSAID - 0.

[Current methods of tear film stability assessment].

Dry eye syndrome (DES) is a multifactorial disease of the ocular surface characterized by a loss of homeostasis of the tear film, and accompanied by ocular symptoms, in which tear film instability and hyperosmolarity, ocular surface inflammation and damage, and neurosensory abnormalities play etiological roles. The clinical manifestations of DES can be highly variable, so its diagnosing is typically based on a combination of symptoms, signs, and clinical tests. Majority of the techniques involved in the diagnosis of this disease has a large degree of subjectivity.

[Polymorphic markers of certain genes in the development of dry keratoconjunctivitis in patients with rheumatoid arthritis and Sjogren's syndrome].

The article reviews literature on relationships between polymorphic variants of the genes THBS1, GTF2I, MUC1, TRIM21, STAT4, PTPN22 with clinical features of dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome. The development and implementation of a method for analyzing polymorphic gene variants used to diagnose dry keratoconjunctivitis in rheumatoid arthritis and Sjogren's syndrome will allow assessment of the possibility of developing dry keratoconjunctivitis and/or its progression in patients with autoimmune diseases or in people at risk. Determination of clinical and morphological regularities of dry keratoconjunctivitis in accordance with the revealed molecular and genetic changes will contribute to better understanding of the etiology and pathogenesis of ophthalmological manifestations of autoimmune diseases, and will also help improve the diagnostics and prognosis of dry keratoconjunctivitis.

[Inflammatory granuloma after intracanalicular punctal plug migration (a clinical case)].

At present, installation of punctal plugs (tear duct occluders) draws attention of ophthalmologists, but this method of treating dry eye syndrome (DES) is not without complications. Considering the rise of DES occurrence - the tendency anticipated to continue - as well as expansion of indications for installation of tear duct occluders, their usage can be expected to rise. The article describes a relatively rare clinical case that involved intracanalicular migration of silicone punctal plug.