Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae.

Background: Adhesion protein E (PE) of Haemophilus influenzae is a 16 - 18 kDa protein with 160 amino acids which causes adhesion to epithelial cells and acts as a major factor in pathogenesis.

Objectives: In this study, we performed cloning, expression and purification of PE as a candidate antigen for vaccine design upon further study.

Materials And Methods: At first, the pe gene of NTHi ATCC 49766 strain (483 bp) was amplified by PCR.