Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea.

A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38 was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38 grew at 15-40 °C (optimum, 30 °C) at pH 6-9 (optimum, pH 7) and at 2-6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38 was closely related to those of J.

Poor Prognostic Effects of Lymphocytopenia Induced by Preoperative Chemoradiotherapy in Rectal Cancer.

Background: We evaluated the prognostic effect of lymphocytopenia caused by preoperative radiation therapy and chemotherapy in patients with rectal cancer.

Methods: A total of 147 patients with rectal cancer who underwent preoperative chemoradiotherapy and surgical resection between 2008 to 2021 participated. Lymphocyte nadir less than 500/μl were defined as lymphocytopenia in this study.

Comparison of the STANDARD M10 . , Xpert . , and BD MAX Cdiff assays as confirmatory tests in a two-step algorithm for diagnosing infection.

Current guidelines recommend a two-step algorithm rather than relying solely on a single test for diagnosing infection. This algorithm starts with enzyme immunoassay (EIA) for detecting glutamate dehydrogenase (GDH) and toxins A/B, followed by nucleic acid amplification test (NAAT) for GDH-positive but toxin-negative cases. This study compared the performance of three commercial NAATs: the STANDARD M10 , Xpert , and BD MAX Cdiff assays, utilized as confirmatory testing of the two-step algorithm.

Clinical evaluation of advanced MALDI-TOF MS for carbapenemase subtyping in Gram-negative isolates.

Unlabelled: The spread of carbapenemase-producing Enterobacterales (CPE) is emerging as a significant clinical concern in tertiary hospitals and, in particular, long-term care facilities with deficiencies in infection control. This study aims to evaluate an advanced matrix-assisted laser desorption/ionization (A-MALDI) mass spectrometry method for the identification of carbapenemases and further discrimination of their subtypes in clinical isolates. The A-MALDI method was employed to detect CPE target proteins.

Dosimetric advantages of dual arc increments for head and neck volumetric-modulated arc therapy in the Monaco treatment planning system.

Purpose: This study aimed to assess the dosimetric advantages of dual arc increments in head and neck volumetric-modulated arc therapy (VMAT) in the Monaco treatment planning system (TPS).

Methods: Three VMAT plans were created for each of the 10 patients by prescribing 70 Gy in 35 fractions with arc increment combinations of 30°/30°, 15°/15°, and 30°/15° in the Monaco TPS. The dose to 0.

Impaired chaperone-mediated autophagy leads to abnormal SORT1 (sortilin 1) turnover and CES1-dependent triglyceride hydrolysis.

SORT1 (sortilin 1), a member of the the Vps10 (vacuolar protein sorting 10) family, is involved in hepatic lipid metabolism by regulating very low-density lipoprotein (VLDL) secretion and facilitating the lysosomal degradation of CES1 (carboxylesterase 1), crucial for triglyceride (TG) breakdown in the liver. This study explores whether SORT1 is targeted for degradation by chaperone-mediated autophagy (CMA), a selective protein degradation pathway that directs proteins containing KFERQ-like motifs to lysosomes via LAMP2A (lysosomal-associated membrane protein 2A). Silencing LAMP2A or HSPA8/Hsc70 with siRNA increased cytosolic SORT1 protein levels.

Consensus in Psychiatric Emergencies Using the Delphi Technique.

Objective: This study aimed to elicit expert consensus on the necessary components of a seclusion room module required to accommodate and manage psychiatric emergency patients requiring both medical and surgical interventions in infectious disease situations.

Methods: A two-round Delphi survey was conducted among 38 medical professionals, architects, and spatial design experts. The survey assessed the effectiveness, feasibility, and urgency of spatial scales, spatial organization, and movement system domains related to the necessary elements of a seclusion room.

Soft Sputtering of Large-Area 2D MoS Layers Using Isolated Plasma Soft Deposition for Humidity Sensors.

2D transition-metal dichalcogenides are emerging as key materials for next-generation semiconductor technologies owing to their tunable bandgaps, high carrier mobilities, and exceptional surface-to-volume ratios. Among them, molybdenum disulfide (MoS) has garnered significant attention. However, scalable wafer-level deposition methods that enable uniform layer-controlled synthesis remain a critical challenge.

Validation of an interferon-gamma enzyme-linked immunosorbent spot assay to evaluate cell-mediated immunity against severe acute respiratory syndrome coronavirus 2.

The COVID-19 pandemic forced the rapid development of methods to measure humoral and cellular immunity against SARS-CoV-2. The lack of a global standardized protocol and the high variability of intra- and inter-assay precision of the T-cell response made it difficult to compare T-cell assay results with those of other laboratories. The interferon-gamma enzyme-linked immunosorbent spot (IFN-γ ELISpot) assay for immunogenicity evaluation was validated using naturally infected donor peripheral blood mononuclear cells, a commercially available IFN-γ ELISpot kit, and a SARS-CoV-2 specific peptide pool.

Integrated analysis of per- and polyfluoroalkyl substances and plasma lipidomics profiles in multi-ethnic Asian subjects for exposome research.

Background: Perfluoroalkyl and polyfluoroalkyl substances (PFAS) exposure has been associated with metabolic diseases, however, the underlying molecular pathogenesis remains to be understood. Integrated PFAS and lipidomic analysis has the potential to identify alterations in lipid metabolism pathways for exposome research.

Methods: A targeted LC-MS/MS method was developed for the quantification of 14 PFAS from human plasma samples (n = 96).

Effectiveness of interventions for convergence insufficiency: an observational study evaluating clinical outcomes and health-related quality of life using AS20.

Background: Convergence insufficiency (CI) is a common condition that can impair visual performance and comfort during close visual work. This observational study evaluated the effectiveness of interventions on clinical outcomes and health-related quality-of-life using the Adult Strabismus-20 (AS20) questionnaire in patients with CI.

Methods: Data was extracted from a database collected at first consultation from 2015 to 2022.

Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations.

Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal editing outcomes. Here we show that the efficiency of delivering RNPs can be enhanced by cell-penetrating peptides (covalently fused to the protein or as excipients) and that lipid nanoparticles (LNPs) encapsulating RNPs can be optimized for enhanced RNP stability, delivery efficiency and editing potency.

A vaccine platform targeting lung-resident memory CD4 T-cells provides protection against heterosubtypic influenza infections in mice and ferrets.

Lung tissue-resident memory T (T) cells induced by influenza vaccination are crucial for heterosubtypic immunity upon re-exposure to the influenza virus, enabling rapid and robust responses upon reactivation. To enhance the efficacy of influenza vaccines, we induce the generation of lung T cells following intranasal vaccination with a commercial influenza vaccine adjuvanted with NexaVant (NVT), a TLR3 agonist-based adjuvant. We demonstrate that intranasal immunization with the NVT-adjuvanted vaccine provides improved protection against influenza virus infections by inducing the generation of CD4 T cells in the lungs in a type I interferon-dependent manner.

Impact of complete revascularization on long-term clinical outcomes in patients with multi-vessel disease, including chronic total occlusion.

To compare the long-term prognostic effect of complete percutaneous coronary intervention (PCI) on cardiovascular events in chronic total occlusion (CTO) patients with the multi-vessel disease (MVD) compared with medical therapy (MT). We enrolled 441 patients with CTO and MVD who underwent PCI. The study population was divided into the CTO-PCI (n = 231) and the CTO-MT (n = 210) groups.

Comparative analysis of treatment modalities for solitary, small (≤3 cm) hepatocellular carcinoma: A systematic review and network meta-analysis of oncologic outcomes.

Background: Solitary hepatocellular carcinoma measuring ≤3 cm represents approximately 30% of hepatocellular carcinoma cases, yet treatment guidelines lack robust evidence. This study compares oncologic outcomes after ablation, liver resection, and liver transplantation for solitary, small hepatocellular carcinoma.

Methods: We systematically searched databases up to 7 February 2022, for studies including adults with solitary hepatocellular carcinoma ≤3 cm treated by any ablation, liver resection, or liver transplantation.