In addition to undesirable odorous gases, substantial amounts of greenhouse gases (GHG), particularly methane (CH), are generated during the storage of livestock manure. To reduce the CH emissions, first, pig slurry (PS) was stored for 40 d at 30 °C after adjusting the pH at 5.0-7.
View Article and Find Full Text PDFTo elucidate the enhanced methane yield from organic wastes, the effects of low-strength ultrasonication on the microbial community structures in upflow anaerobic sludge blanket reactors were for the first time analyzed using pyrosequencing. Interestingly, a more even microbial community was observed in the ultrasonicated granules than in the control, which could compensate for the decreased richness and resulted in comparable (archaea) or even higher (bacteria) diversity. The ultrasonicated granules contained higher levels of δ-Proteobacteria, of which many are reportedly potential syntrophs, as well as methanogenic genera Methanosaeta, Methanotorris, and Methanococcus.
View Article and Find Full Text PDFIn the present work, lactic acid (LA) production performance with granulation was investigated at various hydraulic retention times (HRTs), 8-0.5h. Glucose was used as a feedstock, and anaerobic mixed cultures were inoculated in an up-flow anaerobic sludge blanket reactor.
View Article and Find Full Text PDFIn this study, the positive effects of low-strength ultrasonication (LS-ultrasonication) on the anaerobic digestion (AD) performance were investigated by continuously operating an upflow anaerobic sludge blanket reactor (UASBr) and a dry digester. In the ultrasonicated UASBr system (1 s per min, 0.05 W/mL), ultrasonication enhanced the CH₄ production by 38% and 19% in an ambient and a mesophilic condition, respectively.
View Article and Find Full Text PDFTo elucidate the correlation between enhanced biogas production and changed physico-chemical properties of methanogenic granules after low strength ultrasonication, in this study, the effects of low strength ultrasonication on the settling velocity, permeability, porosity, and fluid collection efficiency of the methanogenic granules were investigated. In addition, their morphological changes were visualized using a scanning electron microscopic technique. The experimental results indicate that low strength ultrasonication increased both the permeability (37%) and specific surface area (230%) of the granules through the generation of greater craters and cracks on the granular surface compared to the control granules.
View Article and Find Full Text PDFThe performance of dry anaerobic digestion (AD) of food waste was investigated under mesophilic conditions and the methanogenic community was investigated using 454 pyrosequencing. Stable dry AD was achieved by hydraulic retention time (HRT) control without the addition of alkali agents. The average CH4 production rate, CH4 content, and volatile solid reduction rate were 2.
View Article and Find Full Text PDFBioresour Technol
September 2012
In this study, low-strength ultrasonication was applied at various ultrasonication densities (UDs) (0-0.1 W/mL) and ultrasonication time (UT) (0-30 min) to methanogenic granules on the purpose of increasing their activity, and eventually, enhancing the performance of upflow anaerobic sludge blanket reactor (UASBr). Batch test results showed that 5 min of ultrasonication at 0.
View Article and Find Full Text PDFWith increasing concerns over the limited capacity of landfills, conservation of resources, and reduction of CO(2) emissions, high-solids (dry) anaerobic digestion of organic solid waste (OSW) is attracting a great deal of attention these days. In the present work, two dry anaerobic co-digestion systems fed with different mixtures of OSW were continuously operated under mesophilic conditions. Dewatered sludge cake was used as a main seeding source.
View Article and Find Full Text PDFThe individual effects of alkaline (pH 8-13) and ultrasonic (3750-45,000kJ/kg TS) pretreatments on the disintegration of sewage sludge were separately tested, and then the effect of combining these two methods at different intensity levels was investigated using response surface methodology (RSM). In the combined pretreatment, ultrasonic treatment was applied to the alkali-pretreated sludge. While the solubilization (SCOD/TCOD) increase was limited to 50% in individual pretreatments, it reached 70% in combined pretreatment, and the results clearly showed that preconditioning of sludge at high pH levels played a crucial role in enhancing the disintegration efficiency of the subsequent ultrasonic pretreatment.
View Article and Find Full Text PDFThis study investigated the effects of food waste constituents on thermophilic (55 degrees C) anaerobic codigestion of sewage sludge and food waste by using statistical techniques based on biochemical methane potential tests. Various combinations of grain, vegetable, and meat as cosubstrate were tested, and then the data of methane potential (MP), methane production rate (MPR), and first-order kinetic constant of hydrolysis (kH) were collected for further analyses. Response surface methodology by the Box-Behnken design can verify the effects and their interactions of three variables on responses efficiently.
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