Synergistic Effects of Nisin, Lysozyme, Lactic Acid, and Citricidal for Enhancing Pressure-Based Inactivation of , , and Endospores.

The inactivation of bacterial endospores continues to be the main curtailment for further adoption of high-pressure processing in intrastate, interstate, and global food commerce. The current study investigated the effects of elevated hydrostatic pressure for the inactivation of endospore suspension of three indicator spore-forming bacteria of concern to the food industry. Additionally, the effects of four bacteriocin/bactericidal compounds were studied for augmenting the decontamination efficacy of the treatment.

Inactivation of Shiga toxin-producing Escherichia coli O157: H7 and mesophilic background microbiota of meat homogenate using elevated hydrostatic pressure, mild heat, and thymol.

A six-strain mixture of E. coli O157:H7 was exposed to 0 to 9 min of six treatments: (i) hydrostatic pressure (400 MPa) at 4 °C; (ii) hydrostatic pressure and thymol at 4 °C; (iii) thymol at 4 °C; (iv) heat at 40 °C; (v) hydrostatic pressure at 40 °C; and (vi) hydrostatic pressure and thymol at 40 °C. Pressure intensity level of 400 MPa and thymol concentration of 0.

Sensitivity of Planktonic Cells of to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid.

Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against . Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% /) were applied for the reduction in four-strain mixture of in HEPES buffer at 4 and 40 °C for up to 7 min.

Fate and Biofilm Formation of Wild-Type and Pressure-Stressed Pathogens of Public Health Concern in Surface Water and on Abiotic Surfaces.

Since the historic outbreak near Broad Street in London, which serves as cornerstone of modern epidemiology, infectious diseases spread in surface and sub-surface water has been a persisting public health challenge. The current study investigated persistence of wild-type and pressure-stressed , O157:H7, and non-typhoidal serovars in surface water stored aerobically for up to 28 days at 5, 25, and 37 °C. Additionally, biofilm formation of wild-type and pressure-stressed non-typhoidal serovars were monitored on surface of stainless steel and rubber coupons for 28 days at 25 and 37 °C.

Augmenting the Pressure-Based Pasteurization of by Synergism with Nisin and Mild Heat.

The current study investigated inactivation using mild heat with elevated hydrostatic pressure and nisin under buffered condition. A four-strain pathogen mixture was exposed to 0 (control) and up to 9 min of (1) 4 °C elevated pressure; (2) 4 °C elevated pressure and nisin; (3) 4 °C nisin; (4) heat at 40 °C; (5) 40 °C elevated pressure; (6) 40 °C elevated pressure and nisin; and (7) 40 °C nisin. Elevated hydrostatic pressure at 400 MPa (Hub880 Explorer, Pressure BioScience Inc.

Interactions of Carvacrol, Caprylic Acid, Habituation, and Mild Heat for Pressure-Based Inactivation of O157 and Non-O157 Serogroups of Shiga Toxin-Producing in Acidic Environment.

The current study investigated synergism of elevated hydrostatic pressure, habituation, mild heat, and antimicrobials for inactivation of O157 and non-O157 serogroups of Shiga toxin-producing Various times at a pressure intensity level of 450 MPa were investigated at 4 and 45 °C with and without carvacrol, and caprylic acid before and after three-day aerobic habituation in blueberry juice. Experiments were conducted in three biologically independent repetitions each consist of two replications and were statistically analyzed as a randomized complete block design study using ANOVA followed by Tukey- and Dunnett's-adjusted mean separations. Under the condition of this experiment, habituation of the microbial pathogen played an influential ( < 0.