Publications by authors named "Sadia Monzur"
Cancer stem cells (CSCs) are small subpopulation sharing similar properties like normal stem such as self-renewal and differentiation potential to direct tumor growth. Last few years, scientists considered CSCs as the cause of phenotypic heterogeneity in diverse cancer types. Also, CSCs contribute to cancer metastasis and recurrence.
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- * Researchers optimized a protocol to produce soluble Cripto-1 protein using a T7 expression system, focusing on the challenges posed by the protein's complex structure due to its 12 cysteine residues.
- * The optimized process included using specific concentrations of IPTG and imidazole for protein expression and purification, achieving over 26.6% recovery of Cripto-1, which then effectively inhibited CSC sphere formation and promoted differentiation.
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- Diphenyleneiodonium (DPI) has been reevaluated as a potential anticancer drug, specifically targeting cancer stem cells (CSCs) which are often resistant to standard chemotherapy.
- The study found that DPI is effective at much lower concentrations (around 5.52 to 12 nM) in three-dimensional culture assays compared to traditional assays, where higher concentrations (712 nM) were required for noticeable effects.
- The research suggests that 3D culture systems may provide a better model for screening anti-CSC drug candidates, as they more accurately replicate the tumor microenvironment compared to 2D culture systems.
Cancer stem cells (CSCs) are subpopulations in the malignant tumors that show self-renewal and multilineage differentiation into tumor microenvironment components that drive tumor growth and heterogeneity. In previous studies, our group succeeded in producing a CSC model by treating mouse induced pluripotent stem cells. In the current study, we investigated the potential of CSC differentiation into blood cells under chemical hypoxic conditions using CoCl.
View Article and Find Full Text PDFMEK1/2 is a bottleneck that induces cancer stem cells to activate the PI3K/AKT pathway.
Biochem Biophys Res Commun
October 2021
Cancer stem cells (CSCs) are responsible for cancer initiation, drug resistance, and aggressive tumor phenotypes. Our lab has established a novel method to induce CSCs from induced pluripotent stem (iPS) cells in a microenvironment mimicking chronic inflammation. The converted cells acquired CSC characteristics and developed malignant tumors.
View Article and Find Full Text PDFWe previously demonstrated the conversion of normal stem cells, including induced pluripotent stem cells (iPSCs), into cancer stem cells (CSCs) without genetic manipulation. Herein, we designed a meta-analysis to assess gene expression profiles in different breast cancer cell lines focusing on the secretory factors responsible for conversion. As a result, fibroblast growth factor 2 (FGF2) was found to be the best candidate in T47D and BT549 cells, of which conditioned medium was previously successful in inducing CSCs.
View Article and Find Full Text PDFBreast cancer is the first common cause of cancer-related death in women worldwide. Since the malignancy and aggressiveness of breast cancer have been correlated with the presence of breast cancer stem cells, the establishment of a disease model with cancer stem cells is required for the development of a novel therapeutic strategy. Here, we aimed to evaluate the availability of cancer stem cell models developed from mouse induced pluripotent stem cells with the conditioned medium of different subtypes of breast cancer cell lines, the hormonal-responsive T47D cell line and the triple-negative breast cancer BT549 cell line, to generate tumor models.
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