Isolation of Chlamydia trachomatis from urethral scrapings of men.

Two strains of Chlamydia were isolated in McCoy cell cultures and hens' yolk sacs from urethral scrapings of men suffering from "nonspecific" urethritis. Their identification as Chlamydia trachomatis was based on cytoplasmic inclusions staining with iodine and on indirect immunofluorescence with anti-LGV serum. Both tests were performed in McCoy cells.

Infection of cattle and livestock handlers with Coxiella burnetti and Chlamydiae in the farm of Bernolákovo (West Slovakia).

Antibodies to phase II C. burnetti and to chlamydial antigen were found in sera of dairy cows and livestock handlers collected in the farm of Bernolákovo [West Slovakia]. The levels of C.

[Cattle vaccination against Q fever in the rural district of Bratislava].

Cattle attendants on two farms in the Outer Bratislava district showed symptoms of Q-fever. The blood of the cows in these farms was found to contain antibodies to Coxiella burnetti and the causative agent of the disease was detected in milk samples of aborting cows by biological assays on hamsters (Mesocricetus auratus). The naturally invaded dairy cows with antibodies in the blood and heifers without antibodies were vaccinated with different doses of inactivated suspension of C.

[Isolation and identification of Clamydia psittaci as the pathogen in enzootic abortion of sheep in eastern Slovakia].

Abortions in ewes occurred on a large scale in three localities in eastern Slovakia. Antibodies to the group-type ornithosis antigen were detected in titres of 1 : 128 to 1 : 2048 in the aborting ewes in the mentioned localities. Suspensions were prepared from the four samples of material, obtained either from the placentae and afterbirths of the aborting ewes or from the tissues of the aborted foetuses.

[Enzootic abortion among ewes in the Roznava district].

In the winter months of the year 1977, 64 dams out of 170 ewes pregnant for the first time miscarried on one farm in the locality G., Roznava district. Antibodies were revealed in 97% dams after the examination of the blood of 170 ewes from the abortion-affected flock; ornithosis antigen was employed for the examination in the complement-fixing reaction.

Immunization against Q-fever of naturally infected dairy cows.

Dairy cows infected naturally with Coxiella burnetii as evidenced either by presence of phase II agglutinating antibodies in the blood or by shedding C. burnetii in the milk, were vaccinated subcutaneously with formalin-killed phase I C. burnetii organisms.

Vaccination of cattle against Q-fever.

Formalin-killed phase I C. burneti organisms containing the protective antigenic component were used for vaccination of heifers against Q fever. None of the vaccinated heifers contracted Q fever, whereas 8 out of 12 (66.

Experimental infection of weanling pigs with A-swine influenza virus. 3. Immunity in piglets farrowed by antibody-bearing dams experimentally infected a year earlier.

Pigs experimentally infected as weanlings with swine influenza virus, as described in previous papers, were bred from when mature. Attempts to isolate virus at parturition from the placenta and from different organs of some of the piglets immediately after birth gave negative results. Antibody levels were determined in the sows and remaining piglets at different times after birth, and the clinical course, immunity and antibody formation were studied in some of the piglets challenged with swine influenza virus 10 days after birth.