Tissue-specific TCF4 triplet repeat instability revealed by optical genome mapping.

Background: Fuchs endothelial corneal dystrophy (FECD) is the most common repeat-mediated disease in humans. It exclusively affects corneal endothelial cells (CECs), with ≤81% of cases associated with an intronic TCF4 triplet repeat (CTG18.1).

Deciphering novel TCF4-driven mechanisms underlying a common triplet repeat expansion-mediated disease.

Fuchs endothelial corneal dystrophy (FECD) is an age-related cause of vision loss, and the most common repeat expansion-mediated disease in humans characterised to date. Up to 80% of European FECD cases have been attributed to expansion of a non-coding CTG repeat element (termed CTG18.1) located within the ubiquitously expressed transcription factor encoding gene, TCF4.

Phenotype and genotype of concurrent keratoconus and Fuchs endothelial corneal dystrophy.

Purpose: To characterise the phenotype and genotype of concurrent keratoconus and Fuchs endothelial corneal dystrophy (KC + FECD).

Methods: We recruited 20 patients with concurrent KC + FECD for a retrospective observational case series from the United Kingdom and the Czech Republic. We compared eight parameters of corneal shape (Pentacam, Oculus) with two groups of age-matched controls who had either isolated keratoconus (KC) or isolated FECD.

Should Patients with Kearns-Sayre Syndrome and Corneal Endothelial Failure Be Genotyped for a Trinucleotide Repeat, Commonly Associated with Fuchs Endothelial Corneal Dystrophy?

The aim of this study was to describe the ocular phenotype in a case with Kearns-Sayre syndrome (KSS) spectrum and to determine if corneal endothelial cell dysfunction could be attributed to other known distinct genetic causes. Herein, genomic DNA was extracted from blood and exome sequencing was performed. Non-coding gene regions implicated in corneal endothelial dystrophies were screened by Sanger sequencing.

CUGC for posterior polymorphous corneal dystrophy (PPCD).

Name of the disease (synonyms) CUGC for posterior polymorphous corneal dystrophy (PPCD).OMIM# of the disease 122000; 609141; 618031.Name of the analysed genes or DNA/chromosome segments OVOL2 (PPCD1); ZEB1 (PPCD3); GRHL2 (PPCD4).

CRISPR/Cas9-targeted enrichment and long-read sequencing of the Fuchs endothelial corneal dystrophy-associated TCF4 triplet repeat.

Purpose: To demonstrate the utility of an amplification-free long-read sequencing method to characterize the Fuchs endothelial corneal dystrophy (FECD)-associated intronic TCF4 triplet repeat (CTG18.1).

Methods: We applied an amplification-free method, utilizing the CRISPR/Cas9 system, in combination with PacBio single-molecule real-time (SMRT) long-read sequencing, to study CTG18.

Symmetry of horizontal and sagittal condylar path angles: an in vivo study.

The specific aims of the study were to determine (1) the mean condylar path inclination (CP) and Bennett angles (BA) in occlusally healthy dentate subjects and (2) whether there was right-left asymmetry of CP and BA. Eccentric movements of three mm and five mm from reference positions were recorded for 45 subjects using a Cadiax system. The mean right CP angles: 48.

Influence of contamination and cleaning on bond strength to modified zirconia.

Objective: To evaluate the influence of contamination and cleaning procedures on shear bond strength (SBS) to modified zirconia surfaces.

Methods: One hundred zirconium-oxide ceramic disks fabricated with a rough modified surface (Nobel Bond), which allows more micromechanical interlocking for adhesive cementation, were divided into five groups. Groups were contaminated with organic (OC; human blood and saliva) and/or inorganic contaminants (IC; type IV dental stone).

Resin-bonded fixed partial dentures with a new modified zirconia surface: a clinical report.

This report describes the clinical application of a new modified zirconia surface. Resin-bonded fixed partial dentures were fabricated for nonprepared abutments, cemented, and evaluated for retention and esthetics. Initial evaluation of this new modified zirconia surface reveals acceptable mechanical and esthetic properties.

Selected mechanical and physical properties and clinical application of a new low-shrinkage composite restoration.

Polymerization shrinkage is a major concern for bonded direct posterior restorations. Recently, a new low-shrinkage composite resin restorative material was developed. However, few data are available regarding clinical manipulation of this composite.

In vitro shear bond strength of dual-curing resin cements to two different high-strength ceramic materials with different surface texture.

Objective: The purpose of this study was to evaluate the shear bond strength (SBS) of two dual-curing resin cements to two high-strength ceramics with different surface texture.

Material And Methods: Composite cylinders were bonded to aluminum oxide (A) and zirconium oxide (Z) ceramic disks with six different surfaces. Investigated surfaces were for both ceramics: dry-pressed Procera Crown (A1, Z1), machined Procera Bridge (A2, Z2), airborne particle abraded Procera bridge (A3, Z3).

The effect of immediate dentin sealing on the marginal adaptation and bond strengths of total-etch and self-etch adhesives.

Statement Of Problem: Sealing ability and bond strengths of total-etch and self-etch dentin adhesives used for immediate dentin sealing have not been assessed and established.

Purpose: The purpose of this study was to determine the effectiveness of immediate dentin sealing (IDS) using total-etch or self-etch dentin adhesives on microleakage and microtensile bond strength.

Material And Methods: Twenty recently extracted molars were selected, and standard MOD inlay preparations were made with the gingival margins located below the cemento-enamel junction.

In vitro comparative bond strength of contemporary self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion.

This study compared shear bond strengths of six self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion. One hundred twenty zirconia samples were air-abraded (group SB; n = 60) or left untreated (group NO). Composite cylinders were bonded to the zirconia samples with either BisCem (BC), Maxcem (MC), G-Cem (GC), RelyX Unicem Clicker (RUC), RelyX Unicem Applicator (RUA), or Clearfil SA Cement (CSA).

Shade-matching abilities of dental laboratory technicians using a commercial light source.

Purpose: The purpose of this study was to evaluate dental laboratory technicians' abilities to match shades using a light-corrective device under conventional laboratory conditions. The variables measured were years experience, gender, and light source.

Materials And Methods: A 14-item shade-matching quiz (SMQ) was field-tested and deemed adequate.

A retrospective case series of implants used to restore partially edentulous patients with implant-supported removable partial dentures: 31-month mean follow-up results.

Objective: The incorporation of dental implants into removable partial dentures is a common clinical practice, but it is poorly documented in the dental literature. The purpose of this retrospective case series study was to evaluate the survival of endosseous dental implants used in restoring partially edentulous patients with implant-supported removable partial dentures (ISRPDs) of different configurations, based on up to 10 years of follow-up.

Method And Materials: The sample consisted of 23 consecutively treated partially edentulous patients, who, between 1996 and 2005, had a total of 44 implants placed in different arch sites and who were treated with ISRPDs.

An in vitro evaluation of the long-term resin bond to a new densely sintered high-purity zirconium-oxide ceramic surface.

Statement Of Problem: Successful long-term bonding to zirconia ceramic remains a challenge, requiring special cements and surface roughening by airborne-particle abrasion, which might negatively affect the ceramic.

Purpose: The purpose of this study was to evaluate the shear bond strength (SBS) of composite resin cylinders to a modified zirconia surface using different luting techniques after thermal cycling.

Material And Methods: Composite resin (TPH3) cylinders with a diameter of 3 mm were bonded to zirconia ceramics (Procera Zirconia) with a modified surface (RZ) and a machined surface (MZ).

Nanoleakage, ultramorphological characteristics, and microtensile bond strengths of a new low-shrinkage composite to dentin after artificial aging.

Objectives: To study the microtensile bond strengths and nanoleakage of low-shrinkage composite to dentin. The null hypotheses tested were (1) aging does not affect the bonding of low-shrinkage composite; (2) there is no difference in microtensile bond strengths and nanoleakage using different bonding strategies.

Methods: 32 extracted molars were assigned to one of four groups: LS System Adhesive (LS, 3M ESPE); dentin etched for 15s with phosphoric acid+LS System Adhesive (LSpa); Adper Single Bond Plus (SB, 3M ESPE); SB+LS Bond (SBLS).

Microtensile bond strengths and scanning electron microscopic evaluation of self-adhesive and self-etch resin cements to intact and etched enamel.

Statement Of Problem: The longevity of indirect adhesively cemented restorations is directly related to the bond quality of resin cements to dental tissues. Bonding to cervical enamel can generate significantly lower bond strengths than bonding to other enamel areas. The effect of enamel pretreatment on bond strengths of self-etch and self-adhesive resin cements to cervical enamel remains undetermined.