Publications by authors named "Sadaf Kalam"

Porosomes are plasma membrane structures in secretory cells that allow transient docking and/or partial fusion of vesicles during which they release their content then disengage. This is referred to as "kiss and run" exocytosis. During early pregnancy, at the time of receptivity, there is a high level of vesicle activity in uterine epithelial cells (UECs).

View Article and Find Full Text PDF

Acidobacteria represents an underrepresented soil bacterial phylum whose members are pervasive and copiously distributed across nearly all ecosystems. Acidobacterial sequences are abundant in soils and represent a significant fraction of soil microbial community. Being recalcitrant and difficult-to-cultivate under laboratory conditions, holistic, polyphasic approaches are required to study these refractive bacteria extensively.

View Article and Find Full Text PDF

The rhizosphere offers a quintessential habitat for the microbial communities and facilitates a variety of plant-microbe interactions. Members of the genus constitute an important group of plant growth promoting rhizobacteria (PGPR), which improve growth and yield of crops. In a total of 60 bacterial isolates from the tomato rhizosphere, 7 isolates were selected based on distinct morphological characteristics and designated as tomato rhizosphere (TRS) isolates with a number suffixed .

View Article and Find Full Text PDF

Luminal uterine epithelial cells (UEC) have a surge in vesicular activity during early uterine receptivity. It has been predicted these vesicles exit the UEC via exocytosis resulting in secretion and membrane trafficking. The present study investigated the changes in SNARE proteins VAMP2 (v-SNARE) and syntaxin 3 (t-SNARE) localisation and abundance in UECs during early pregnancy in the rat.

View Article and Find Full Text PDF

The drug compliance and its associated factors were investigated among hypertensive patients attending the outpatient department of National Institute of Cardiovascular Disease (NICVD), Karachi, from September 2014 to March 2015. Data were collected retrospectively from 300 hypertensive patients taken treatment for >1 year. Half of the patients (51%) were >55 years of age with the predominance of males (52.

View Article and Find Full Text PDF

For the development of uterine receptivity, many morphological and molecular changes occur in the apical surface of luminal uterine epithelial cells (UECs) including an increase in vesicular activity. Vesicular movements for exocytosis and endocytosis are dependent on microtubules; however, changes in microtubules in UECs during early pregnancy have received little attention. β-tubulin, one of the main component of microtubules, is distributed throughout the cytoplasm of UECs on day 1 (non-receptive) of pregnancy in the rat.

View Article and Find Full Text PDF

Rhizosphere microbial community has diverse metabolic capabilities and plays a crucial role in maintaining plant health. Oligotrophic plant growth promoting rhizobacteria (PGPR), along with difficult-to-culture microbial fractions, might be involved synergistically in microbe-microbe and plant-microbe interactions in the rhizosphere. Among the difficult-to-culture microbial fractions, Acidobacteria constitutes the most dominant phylum thriving in rhizospheric soils.

View Article and Find Full Text PDF

A total of 74 morphologically distinct bacterial colonies were selected during isolation of bacteria from different parts of tomato plant (rhizoplane, phylloplane and rhizosphere) as well as nearby bulk soil. The isolates were screened for plant growth promoting (PGP) traits such as production of indole acetic acid, siderophore, chitinase and hydrogen cyanide as well as phosphate solubilization. Seven isolates viz.

View Article and Find Full Text PDF