Publications by authors named "Sachithra S Ranaweera"
Article Synopsis
- This study investigates how hesperidin (HES) affects diabetes management by analyzing gene expression in high fat diet (HFD)-induced obese mice.
- It was found that HES treatment improved glucose uptake, decreased blood glucose and insulin levels, and enhanced both glucose and insulin tolerance to normal levels.
- Additionally, RNA sequencing revealed that HES restored the expression of key genes involved in insulin signaling and glucose metabolism, contributing to its potential anti-diabetic properties.
Article Synopsis
- Flavonoids, natural compounds found in citrus fruits, have shown promise in reducing fat cell formation (anti-adipogenic effects), although the exact mechanisms remain unclear.
- This study focused on five main citrus flavonoids (hesperidin, narirutin, nobiletin, sinensetin, and tangeretin) to examine their impact on AMPK activation in liver cells treated with palmitate.
- Results indicated that these flavonoids decreased fat accumulation, increased glucose uptake, and activated key proteins involved in fat metabolism, suggesting they could be effective activators of AMPK, which may help reduce fat buildup in cells.
Article Synopsis
- Naringin and naringenin, flavonoids from citrus fruits, have potential anti-diabetic effects, but their mechanisms are not fully understood.
- This study focused on how these compounds affect glucose uptake through AMPK phosphorylation in HepG2 cells treated with high glucose.
- Results showed that both naringin and naringenin boost glucose uptake by promoting AMPK activation and GSK3β phosphorylation, regardless of insulin presence, with naringin having a particularly strong binding affinity to AMPK.
Article Synopsis
- The study investigated the anti-obesity effects of sulforaphane (SFN) and glucoraphanin (GRN) found in broccoli leaf extract (BLE) on fat cells (3T3-L1 adipocytes) and genetically modified obese mice (ob/ob).
- Findings showed that SFN and BLE significantly decreased fat storage and triglyceride levels in both the cells and the mice.
- Additionally, treatment with SFN and BLE improved lipid metabolism by altering the expression of several key genes and reducing fat-related blood markers, indicating a potential therapeutic role against obesity.
Article Synopsis
- * Methods included measuring lipid accumulation, triglyceride content, glucose uptake, and analyzing gene expression changes in 3T3-L1 adipocytes treated with these flavonoids.
- * Findings suggested that both compounds significantly reduce lipid accumulation and alter the expression of key genes in lipid metabolism, indicating their potential as anti-obesity agents.
Background: Sulforaphane (SFN) is an isothiocyanate compound present in cruciferous vegetables. Although the anti-inflammatory effects of SFN have been reported, the precise mechanism related to the inflammatory genes is poorly understood.
Objectives: This study examined the relationship between the anti-inflammatory effects of SFN and the differential gene expression pattern in SFN treated ob/ob mice.